BMRBj - BMREntryMaster

Found 1 Document (1.148 seconds)

BMRB: 17407

2LP0

The complex structure of homeodomain in solution

Authors

LIU, C., ZHOU, B., ZHU, G.

Assembly

homeodomain

Entity

1. homeodomain, entity 1 (polymer, Thiol state: not present), 60 monomers, 7722.073 Da Detail

TRKKRCPYTK YQTLELEKEF LFNMYLTRDR RYEVARVLNL TERQVKIWFQ NRRMKMKKMN

2. homeodomain, entity 2 (polymer, Thiol state: not present), 20 monomers, 2330.236 Da Detail

NQEFDSEEET VEDSLVEDSE

Total weight

10052.31 Da

Max. entity weight

7722.073 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (872 of 1026)	88.1 % (483 of 548)	77.4 % (301 of 389)	98.9 % (88 of 89)
Backbone	97.9 % (468 of 478)	100.0 % (159 of 159)	95.8 % (230 of 240)	100.0 % (79 of 79)
Sidechain	75.8 % (476 of 628)	83.3 % (324 of 389)	62.4 % (143 of 229)	90.0 % (9 of 10)
Aromatic	57.1 % (48 of 84)	73.8 % (31 of 42)	39.0 % (16 of 41)	100.0 % (1 of 1)
Methyl	92.4 % (61 of 66)	100.0 % (33 of 33)	84.8 % (28 of 33)

1. entity 1

TRKKRCPYTK YQTLELEKEF LFNMYLTRDR RYEVARVLNL TERQVKIWFQ NRRMKMKKMN

2. entity 2

NQEFDSEEET VEDSLVEDSE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2LP0, Strand ID: A, B Detail

Release date

2012-06-03

Citation

Structural basis for homeodomain recognition by the cell-cycle regulator Geminin
Zhou, B., Liu, C., Xu, Z., Zhu, G.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 8931-8936, PubMed 22615398 , DOI 10.1073/pnas.1200874109 , Abstract

Related entities 1. homeodomain, entity 1, : 4 : 11 : 85 entities Detail

Related entities 2. homeodomain, entity 2, : 1 : 3 : 5 entities Detail

Interaction partners 2. homeodomain, entity 2, : 17 interactors Detail

More details for 17 interactors identified by 10 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

3 3D HNCO

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D HCCH-COSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

10 3D 1H-15N TOCSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

11 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	1.0 mM
3	potassium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

12 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

13 3D 13C-filtered 13C-edited NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	entity_1	[U-99% 13C; U-99% 15N]	0.80 mM
9	entity_2	natural abundance	1.0 mM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	D2O	natural abundance	100 %

14 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

15 2D 1H-13C HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

16 3D HNCO

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

17 3D CBCA(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

18 3D HNCACB

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

19 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

20 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
15	entity_1	natural abundance	1.0 mM
16	potassium phosphate	natural abundance	25 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	5 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

nullKeywords homeodomain

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Found 1 Document (1.148 seconds)

BMRB: 17407

Sample #1

Sample #2

Sample #3

Related entities 1. homeodomain, entity 1, : 4 : 11 : 85 entities Detail

Related entities 2. homeodomain, entity 2, : 1 : 3 : 5 entities Detail

Interaction partners 2. homeodomain, entity 2, : 17 interactors Detail

Experiments performed 20 experiments Detail

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