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BMRB: 17412

2L8J

GABARAPL-1 NBR1-LIR complex structure

Authors

Rogov, V.V., Rozenknop, A., Rogova, N.Y., Loehr, F., Guentert, P., Dikic, I., Doetsch, V.

Assembly

GABARAPL-1 and NBR1-LIR complex

Entity

1. GABARAPL-1 (polymer, Thiol state: not present), 119 monomers, 14244.98 Da Detail

GSPEFKFQYK EDHPFEYRKK EGEKIRKKYP DRVPVIVEKA PKARVPDLDK RKYLVPSDLT VGQFYFLIRK RIHLRPEDAL FFFVNNTIPP TSATMGQLYE DNHEEDYFLY VAYSDESVY

2. NBR1-LIR (polymer, Thiol state: not present), 18 monomers, 1840.015 Da Detail

GAMGSASSED YIIILPES

Total weight

16084.995 Da

Max. entity weight

14244.98 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 80.8 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.8 % (1360 of 1684)	83.5 % (739 of 885)	76.2 % (508 of 667)	85.6 % (113 of 132)
Backbone	84.6 % (677 of 800)	87.7 % (236 of 269)	81.7 % (331 of 405)	87.3 % (110 of 126)
Sidechain	78.3 % (795 of 1015)	81.3 % (501 of 616)	74.3 % (292 of 393)	33.3 % (2 of 6)
Aromatic	57.4 % (109 of 190)	76.8 % (73 of 95)	37.9 % (36 of 95)
Methyl	92.9 % (117 of 126)	95.2 % (60 of 63)	90.5 % (57 of 63)

1. GABARAPL-1

GSPEFKFQYK EDHPFEYRKK EGEKIRKKYP DRVPVIVEKA PKARVPDLDK RKYLVPSDLT VGQFYFLIRK RIHLRPEDAL FFFVNNTIPP TSATMGQLYE DNHEEDYFLY VAYSDESVY

2. NBR1-LIR

GAMGSASSED YIIILPES

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	GABARAPL-1	[U-98% 15N]	0.6 (±0.05) mM
2	NBR1-LIR	natural abundance	0.9 (±0.05) mM
3	sodium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	4.6 mM
6	protease inhibitor cocktail	natural abundance	1 mM
7	DSS	natural abundance	0.3 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	GABARAPL-1	[U-98% 13C; U-98% 15N]	0.6 (±0.05) mM
11	NBR1-LIR	natural abundance	0.9 (±0.05) mM
12	sodium phosphate	natural abundance	50 mM
13	sodium chloride	natural abundance	100 mM
14	sodium azide	natural abundance	4.6 mM
15	protease inhibitor cocktail	natural abundance	1 mM
16	DSS	natural abundance	0.3 mM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
19	GABARAPL-1	natural abundance	0.9 (±0.05) mM
20	NBR1-LIR	[U-98% 15N]	0.6 (±0.05) mM
21	sodium phosphate	natural abundance	50 mM
22	sodium chloride	natural abundance	100 mM
23	sodium azide	natural abundance	4.6 mM
24	protease inhibitor cocktail	natural abundance	1 mM
25	DSS	natural abundance	0.3 mM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
28	GABARAPL-1	natural abundance	0.9 (±0.05) mM
29	NBR1-LIR	[U-98% 13C; U-98% 15N]	0.6 (±0.05) mM
30	sodium phosphate	natural abundance	50 mM
31	sodium chloride	natural abundance	100 mM
32	sodium azide	natural abundance	4.6 mM
33	protease inhibitor cocktail	natural abundance	1 mM
34	DSS	natural abundance	0.3 mM
35	H2O	natural abundance	95 %
36	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2L8J, Strand ID: A, B Detail

Release date

2011-06-01

Citation

Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1
Rozenknop, A., Rogov, V.V., Rogova, N., Lohr, F., Guntert, P., Dikic, I., Dotsch, V.
J. Mol. Biol. (2011), 410, 477-487, PubMed 21620860 , DOI 10.1016/j.jmb.2011.05.003 , Abstract

Related entities 1. GABARAPL-1, : 3 : 74 entities Detail

Related entities 2. NBR1-LIR, : 1 : 7 entities Detail

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC