NMR STRUCTURE OF THE CYTIDINE REPRESSOR DNA BINDING DOMAIN IN PRESENCE OF OPERATOR HALF-SITE DNA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 70.0 % (540 of 771) | 73.8 % (302 of 409) | 60.1 % (175 of 291) | 88.7 % (63 of 71) |
Backbone | 81.3 % (322 of 396) | 93.2 % (124 of 133) | 67.8 % (135 of 199) | 98.4 % (63 of 64) |
Sidechain | 64.1 % (282 of 440) | 64.5 % (178 of 276) | 66.2 % (104 of 157) | 0.0 % (0 of 7) |
Aromatic | 25.0 % (2 of 8) | 50.0 % (2 of 4) | 0.0 % (0 of 4) | |
Methyl | 92.9 % (65 of 70) | 91.4 % (32 of 35) | 94.3 % (33 of 35) |
1. HTH-TYPE TRANSCRIPTIONAL REPRESSOR CYTR
MKAKKQETAA TMKDVALKAK VSTATVSRAL MNPDKVSQAT RNRVEKAARE VGYLPQPMGR NVKRNESSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.411 MM [U-15N] CYTR DBD, 1.14 MM ATTTATGCAACGCA DNA, 50 MM SODIUM PHOSPHATE, 30 MM SODIUM CHLORIDE, 1 MM EDTA, 90% H2O/10 D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HTH-TYPE TRANSCRIPTIONAL REPRESSOR CYTR | [U-15N] | 0.411 mM | |
2 | ATTTATGCAACGCA DNA | natural abundance | 1.14 mM | |
3 | sodium phosphate | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 30 mM | |
5 | EDTA | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | HTH-TYPE TRANSCRIPTIONAL REPRESSOR CYTR | [U-13C; U-15N] | 1 mM | |
7 | ATTTATGCAACGCA DNA | natural abundance | 1.2 mM | |
8 | sodium phosphate | natural abundance | 50 mM | |
9 | sodium chloride | natural abundance | 30 mM | |
10 | EDTA | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.4 MM [U-15N] CYT DBD, 0.6 MM ATTTATGCAACGCA DNA, 50 MM SODIUM PHOSPHATE, 30 MM SODIUM CHLORIDE, 1 MM EDTA, 4 % C12E5 PEG/HEXANOL, 90% H2O/10%
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HTH-TYPE TRANSCRIPTIONAL REPRESSOR CYTR | [U-15N] | 0.4 mM | |
12 | ATTTATGCAACGCA DNA | natural abundance | 0.6 mM | |
13 | sodium phosphate | natural abundance | 50 mM | |
14 | sodium chloride | natural abundance | 30 mM | |
15 | EDTA | natural abundance | 1 mM | |
16 | C12E5 PEG/HEXANOL | natural abundance | 4 % |