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BMRB: 17420

2L8O

Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148

Authors

Liu, Y., Lee, D., Ciccosanti, C., Nair, L., Rost, B., Acton, T., Xiao, R., Everett, J., Montelione, G., Prestegard, J.

Assembly

Chr148

Entity

1. Chr148 (polymer, Thiol state: not present), 144 monomers, 16391.93 Da Detail

GNKITVEVTV YAAIEKVWKY WNEPAHIMKW CQASPEWHVP AAQNDLKAGG TFTTTMAAKD GSMSFDFGGV YDQVKTNDLI EYTIGDGRKV RIVFTHTGDT TNIVESFDPE ETNPRELQQS GWQAILNSFK SYTENNLEHH HHHH

Formula weight

16391.93 Da

Source organism

Cytophaga hutchinsonii

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 80.4 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.4 % (1345 of 1673)	74.4 % (640 of 860)	85.6 % (560 of 654)	91.2 % (145 of 159)
Backbone	94.7 % (809 of 854)	92.8 % (272 of 293)	95.5 % (403 of 422)	96.4 % (134 of 139)
Sidechain	69.8 % (665 of 953)	64.9 % (368 of 567)	78.1 % (286 of 366)	55.0 % (11 of 20)
Aromatic	39.3 % (77 of 196)	39.8 % (39 of 98)	35.5 % (33 of 93)	100.0 % (5 of 5)
Methyl	95.0 % (133 of 140)	98.6 % (69 of 70)	91.4 % (64 of 70)

1. Chr148

GNKITVEVTV YAAIEKVWKY WNEPAHIMKW CQASPEWHVP AAQNDLKAGG TFTTTMAAKD GSMSFDFGGV YDQVKTNDLI EYTIGDGRKV RIVFTHTGDT TNIVESFDPE ETNPRELQQS GWQAILNSFK SYTENNLEHH HHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Chr148	natural abundance	1 mM
2	sodium azide	natural abundance	3 mM
3	DTT	natural abundance	10 mM
4	calcium chloride	natural abundance	5 mM
5	sodium chloride	natural abundance	200 mM
6	MES	natural abundance	20 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L8O, Strand ID: A Detail

Release date

2011-02-03

Citation

Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148
Liu, Y., Lee, D., Ciccosanti, C., Nair, L., Rost, B., Acton, T., Xiao, R., Everett, J., Montelione, G., Prestegard, J.
Not known

Related entities 1. Chr148, : 1 : 5 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC