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BMRB: 17425

2L8T

Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination

Authors

Dearborn, A.D., Spilman, M.S., Damle, P.K., Chang, J.R., Monroe, E.B., Saad, J.S., Christie, G.E., Dokland, T.

Assembly

island 1 protein gp6

Entity

1. island 1 protein gp6 (polymer, Thiol state: not present), 71 monomers, 8140.964 Da Detail

ETKYELNNTK KVANAFGLNE EDTNLLINAV DLDIKNNMQE ISSELQQSEQ SKQKQYGTTL QNLAKQNRII K

Formula weight

8140.964 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 76.1 %, Completeness (bb): 80.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.1 % (653 of 858)	78.6 % (357 of 454)	71.7 % (226 of 315)	78.7 % (70 of 89)
Backbone	80.5 % (343 of 426)	91.0 % (131 of 144)	69.2 % (146 of 211)	93.0 % (66 of 71)
Sidechain	75.0 % (376 of 501)	72.9 % (226 of 310)	84.4 % (146 of 173)	22.2 % (4 of 18)
Aromatic	69.2 % (18 of 26)	69.2 % (9 of 13)	69.2 % (9 of 13)
Methyl	89.7 % (70 of 78)	84.6 % (33 of 39)	94.9 % (37 of 39)

1. entity

ETKYELNNTK KVANAFGLNE EDTNLLINAV DLDIKNNMQE ISSELQQSEQ SKQKQYGTTL QNLAKQNRII K

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	200 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	entity	[U-99% 13C; U-99% 15N]	1 mM
7	sodium phosphate	natural abundance	10 mM
8	sodium chloride	natural abundance	200 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.3 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.3 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L8T, Strand ID: A, B Detail

Release date

2011-08-16

Citation

The Staphylococcus aureus pathogenicity island 1 protein gp6 functions as an internal scaffold during capsid size determination
Dearborn, A.D., Spilman, M.S., Damle, P.K., Chang, J.R., Monroe, E.B., Saad, J.S., Christie, G.E., Dokland, T.
J. Mol. Biol. (2011), 412, 710-722, PubMed 21821042 , DOI 10.1016/j.jmb.2011.07.036 , Abstract

Entries sharing articles EMDB: 1 entries Detail

EMDB: 1869 released on 2012-03-15
Title Procapsid of Staphylococcus aureus Pathogenicity Island 1