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BMRB: 17431

2L8Y

Solution structure of the E. coli outer membrane protein RcsF (periplasmatic domain)

Authors

Rogov, V.V., Doetsch, V., Rogova, N.Y., Bernhard, F., Loehr, F.

Assembly

outer membrane protein RcsF

Entity

1. outer membrane protein RcsF (polymer, Thiol state: all disulfide bound), 105 monomers, 11121.65 Da Detail

MAPQPKAEPA KPKAPRATPV RIYTNAEELV GKPFRDLGEV SGDSCQASNQ DSPPSIPTAR KRMQINASKM KANAVLLHSC EVTSGTPGCY RQAVCIGSAL NITAK

Formula weight

11121.65 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS45:SG	1:CYS89:SG
2	disulfide	sing	1:CYS80:SG	1:CYS95:SG

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 86.0 %, Completeness (bb): 90.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.0 % (999 of 1161)	83.0 % (507 of 611)	91.0 % (406 of 446)	82.7 % (86 of 104)
Backbone	90.0 % (547 of 608)	87.8 % (180 of 205)	91.9 % (284 of 309)	88.3 % (83 of 94)
Sidechain	82.1 % (535 of 652)	79.1 % (321 of 406)	89.4 % (211 of 236)	30.0 % (3 of 10)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	92.3 % (96 of 104)	88.5 % (46 of 52)	96.2 % (50 of 52)

1. RcsF

MAPQPKAEPA KPKAPRATPV RIYTNAEELV GKPFRDLGEV SGDSCQASNQ DSPPSIPTAR KRMQINASKM KANAVLLHSC EVTSGTPGCY RQAVCIGSAL NITAK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RcsF(31-134)	[U-98% 13C; U-98% 15N]	0.6 (±0.05) mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	protease inhibitors cocktail	natural abundance	4.6 mM
5	DSS	natural abundance	0.3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2L8Y, Strand ID: A Detail

Release date

2011-04-07

Citation

A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF
Rogov, V.V., Rogova, N., Bernhard, F., Lohr, F., Dotsch, V.
J. Biol. Chem. (2011), 286, 18775-18783, PubMed 21471196 , DOI 10.1074/jbc.M111.230185 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P69411 released on 2005-03-01
Title RCSF_ECOLI Entity Outer membrane lipoprotein RcsF

Related entities 1. outer membrane protein RcsF, : 1 : 4 entities Detail

Interaction partners 1. outer membrane protein RcsF, : 8 interactors Detail

More details for 8 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC