BMRB: 17434

solution structure of the C-terminal domain of H-NS like protein Bv3F

Authors

Li, Y., Xia, B.

Assembly

C-terminal domain of Bv3F

Entity

1. C-terminal domain of Bv3F (polymer), 44 monomers, 4823.443 Da Detail

MSTVPKYRDP ATGKTWSGRG RQPAWLGNDP AAFLIQPDLP AILE

Formula weight

4823.443 Da

Source organism

Burkholderia vietnamiensis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (472 of 509)	91.0 % (243 of 267)	95.0 % (189 of 199)	93.0 % (40 of 43)
Backbone	94.4 % (238 of 252)	96.5 % (83 of 86)	93.8 % (120 of 128)	92.1 % (35 of 38)
Sidechain	92.3 % (274 of 297)	88.4 % (160 of 181)	98.2 % (109 of 111)	100.0 % (5 of 5)
Aromatic	97.6 % (41 of 42)	100.0 % (21 of 21)	94.7 % (18 of 19)	100.0 % (2 of 2)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. Bv3F

MSTVPKYRDP ATGKTWSGRG RQPAWLGNDP AAFLIQPDLP AILE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of H-NS like protein Bv3F	[U-13C, >90%; U-15 N, >90%]		0.7 mM
2	sodium phosphate	[U-100% 13C; U-100% 15N]		50 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L92, Strand ID: A Detail

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Release date

2015-06-22

Citation

Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins
Gordon, B.R.G., Li, Y., Cote, A., Weirauch, M.T., Ding, P., Hughes, T.R., Navarre, W.W., Xia, B., Liu, J.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 10690-10695, PubMed 21673140 , DOI 10.1073/pnas.1102544108 , Abstract

Related entities 1. C-terminal domain of Bv3F, : 1 : 5 entities Detail

Experiments performed 1 experiments Detail