BMRB: 17435

solution structure of the C-terminal domain of Salmonella H-NS

Authors

Li, Y.

Assembly

C-terminal domain of Salmonella H-NS

Entity

1. C-terminal domain of Salmonella H-NS (polymer, Thiol state: not present), 49 monomers, 5556.172 Da Detail

AARPAKYSYV DENGETKTWT GQGRTPAVIK KAMEEQGKQL EDFLIKELE

Formula weight

5556.172 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.7 %, Completeness (bb): 83.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.7 % (526 of 580)	97.1 % (297 of 306)	80.2 % (178 of 222)	98.1 % (51 of 52)
Backbone	83.4 % (242 of 290)	97.0 % (97 of 100)	69.2 % (99 of 143)	97.9 % (46 of 47)
Sidechain	98.2 % (329 of 335)	97.1 % (200 of 206)	100.0 % (124 of 124)	100.0 % (5 of 5)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (18 of 18)	100.0 % (1 of 1)
Methyl	97.8 % (45 of 46)	95.7 % (22 of 23)	100.0 % (23 of 23)

1. H-NS

AARPAKYSYV DENGETKTWT GQGRTPAVIK KAMEEQGKQL EDFLIKELE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Salmonella H-NS	[U-13C, >90%; U-15 N, >90%]		0.7 mM
2	sodium phosphate	[U-100% 13C; U-100% 15N]		50 mM

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L93, Strand ID: A Detail

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Release date

2015-06-22

Citation

Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins
Gordon, B.R.G., Li, Y., Cote, A., Weirauch, M.T., Ding, P., Hughes, T.R., Navarre, W.W., Xia, B., Liu, J.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 10690-10695, PubMed 21673140 , DOI 10.1073/pnas.1102544108 , Abstract

Related entities 1. C-terminal domain of Salmonella H-NS, : 1 : 2 : 20 entities Detail

Interaction partners 1. C-terminal domain of Salmonella H-NS, : 3 interactors Detail

Experiments performed 1 experiments Detail