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BMRB: 17443

2Y4W

Solution structure of human ubiquitin conjugating enzyme Rad6b

Authors

Huang, A., Hibbert, R.G., Dejong, R.N., Das, D., Sixma, T.K., Boelens, R.

Assembly

Solution structure of human ubiquitin conjugating enzyme Rad6b

Entity

1. Solution structure of human ubiquitin conjugating enzyme Rad6b (polymer, Thiol state: all free), 152 monomers, 17312.14 Da Detail

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

Formula weight

17312.14 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 87.9 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.9 % (1580 of 1797)	89.9 % (850 of 945)	84.7 % (586 of 692)	90.0 % (144 of 160)
Backbone	95.0 % (840 of 884)	95.9 % (284 of 296)	94.0 % (423 of 450)	96.4 % (133 of 138)
Sidechain	82.6 % (875 of 1059)	86.3 % (560 of 649)	78.4 % (304 of 388)	50.0 % (11 of 22)
Aromatic	41.3 % (62 of 150)	69.3 % (52 of 75)	9.7 % (7 of 72)	100.0 % (3 of 3)
Methyl	99.3 % (135 of 136)	100.0 % (68 of 68)	98.5 % (67 of 68)

1. UBIQUITIN-CONJUGATING ENZYME E2 B

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

Show sample condition

Sample

Solvent system 50mM KH2PO4/K2HPO4 (pH 8.0), 300 mM NaCl, 1 mM DTT, 95%/5% H2O/D2O, Pressure 1.000 atm, Temperature 310.000 K, pH 8.000, Details 1 mM

#	Name	Isotope labeling	Type	Concentration
1	UBIQUITIN-CONJUGATING ENZYME E2 B			1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl proton	internal	indirect	0.2514495
¹H	DSS	methyl proton	internal	indirect	1.0
¹⁵N	DSS	methyl proton	internal	indirect	0.1013291

Referencing offset: 2.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl proton	internal	indirect	0.2514495
¹H	DSS	methyl proton	internal	indirect	1.0
¹⁵N	DSS	methyl proton	internal	indirect	0.1013291

Referencing offset: 2.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl proton	internal	indirect	0.2514495
¹H	DSS	methyl proton	internal	indirect	1.0
¹⁵N	DSS	methyl proton	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl proton	internal	indirect	0.2514495
¹H	DSS	methyl proton	internal	indirect	1.0
¹⁵N	DSS	methyl proton	internal	indirect	0.1013291

Referencing offset: 2.72 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2Y4W, Strand ID: A Detail

Release date

2011-05-11

Citation

Symmetry and asymmetry of the RING-RING dimer of Rad18
Huang, A., Hibbert, R.G., de Jong, R.N., Das, D., Sixma, T.K., Boelens, R.
J. Mol. Biol. (2011), 410, 424-435, PubMed 21549715 , DOI 10.1016/j.jmb.2011.04.051 , Abstract

Related entities 1. Solution structure of human ubiquitin conjugating enzyme Rad6b, : 1 : 7 : 289 entities Detail

Interaction partners 1. Solution structure of human ubiquitin conjugating enzyme Rad6b, : 11 interactors Detail

More details for 11 interactors identified by 3 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC