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BMRB: 17451

2L9J

Central domain of RSV M2-1

Authors

Dubosclard, V., Blondot, M., Eleouet, J., Bontems, F., Sizun, C.

Assembly

M2-1

Entity

1. M2-1 (polymer, Thiol state: all free), 121 monomers, 13494.25 Da Detail

GSEISGAAEL DRTEEYALGV VGVLESYIGS INNITKQSAC VAMSKLLTEL NSDDIKKLRD NEEPNSPKIR VYNTVISYIE SNRKNNKQTI HLLKRLPADV LKKTIKNTLD IHKSITINNP K

Formula weight

13494.25 Da

Source organism

Pneumovirus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 97.9 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.9 % (1389 of 1419)	97.8 % (727 of 743)	97.8 % (533 of 545)	98.5 % (129 of 131)
Backbone	97.1 % (697 of 718)	97.1 % (236 of 243)	96.6 % (346 of 358)	98.3 % (115 of 117)
Sidechain	98.8 % (807 of 817)	98.2 % (491 of 500)	99.7 % (302 of 303)	100.0 % (14 of 14)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (156 of 156)	100.0 % (78 of 78)	100.0 % (78 of 78)

1. M2-1

GSEISGAAEL DRTEEYALGV VGVLESYIGS INNITKQSAC VAMSKLLTEL NSDDIKKLRD NEEPNSPKIR VYNTVISYIE SNRKNNKQTI HLLKRLPADV LKKTIKNTLD IHKSITINNP K

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	M2-1	[U-100% 13C; U-100% 15N]	0.13 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	93 %
6	D2O	natural abundance	7 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	M2-1	[U-100% 13C]	0.1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	D2O	natural abundance	100 %

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details c12e5/hexanol

#	Name	Isotope labeling	Concentration
12	M2-1	[U-100% 15N]	0.2 mM
13	sodium phosphate	natural abundance	50 mM
14	sodium chloride	natural abundance	150 mM
15	DTT	natural abundance	1 mM
16	c12e5	natural abundance	0.055 v/v
17	Hexanol	natural abundance	0.017 v/v
18	H2O	natural abundance	93 %
19	D2O	natural abundance	7 %

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details polyacrylamide gel

#	Name	Isotope labeling	Concentration
20	M2-1	[U-100% 15N]	0.12 mM
21	sodium phosphate	natural abundance	50 mM
22	sodium chloride	natural abundance	150 mM
23	DTT	natural abundance	1 mM
24	acrylamide/bisacryamide	natural abundance	6 %
25	H2O	natural abundance	93 %
26	D2O	natural abundance	7 %

Sample #5

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
27	M2-1	[U-100% 15N]	0.12 mM
28	sodium phosphate	natural abundance	50 mM
29	sodium chloride	natural abundance	150 mM
30	DTT	natural abundance	1 mM
31	H2O	natural abundance	93 %
32	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L9J, Strand ID: A Detail

RDC

100 RDC values in 2 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

Release date

2011-05-02

Citation

1H, 13C, and 15N resonance assignment of the central domain of hRSV transcription antitermination factor M2-1
Dubosclard, V., Blondot, M., Eleouet, J., Bontems, F., Sizun, C.
Biomol. NMR Assign. (2011), 5, 237-239, PubMed 21523439 , DOI 10.1007/s12104-011-9308-3 , Abstract

Related entities 1. M2-1, : 1 : 6 : 13 entities Detail

Interaction partners 1. M2-1, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC