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BMRB: 17456

2LAU

1H, 15N, 13C chemical shift assignment of the THAP domain 1-81 from the cell growth suppressor human THAP11 protein

Authors

Durand, J., Milon, A., Gervais, V.

Assembly

THAP domain of human THAP11

Entity

1. THAP domain (polymer, Thiol state: free and other bound), 81 monomers, 9112.320 Da Detail

GSPGFTCCVP GCYNNSHRDK ALHFYTFPKD AELRRLWLKN VSRAGVSGCF STFQPTTGHR LCSVHFQGGR KTYTVRVPTI F

2. ZN (non-polymer), 65.409 Da

Total weight

9177.7295 Da

Max. entity weight

9112.32 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS7:SG	2:ZN1:ZN
2	na	sing	1:CYS12:SG	2:ZN1:ZN
3	na	sing	1:CYS62:SG	2:ZN1:ZN
4	na	sing	1:HIS65:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 93.4 %, Completeness (bb): 93.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.4 % (880 of 942)	94.1 % (459 of 488)	92.7 % (345 of 372)	92.7 % (76 of 82)
Backbone	93.9 % (447 of 476)	96.4 % (159 of 165)	92.8 % (218 of 235)	92.1 % (70 of 76)
Sidechain	93.7 % (505 of 539)	92.9 % (300 of 323)	94.8 % (199 of 210)	100.0 % (6 of 6)
Aromatic	86.1 % (105 of 122)	86.9 % (53 of 61)	85.0 % (51 of 60)	100.0 % (1 of 1)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. THAP domain

GSPGFTCCVP GCYNNSHRDK ALHFYTFPKD AELRRLWLKN VSRAGVSGCF STFQPTTGHR LCSVHFQGGR KTYTVRVPTI F

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	THAP domain	[U-99% 13C; U-99% 15N]	0,5 mM
2	Tris-HCl	natural abundance	50 mM
3	sodium chloride	natural abundance	30 mM
4	DTT	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	THAP domain	natural abundance	0,7 mM
6	Tris-HCl	natural abundance	50 mM
7	sodium chloride	natural abundance	30 mM
8	DTT	natural abundance	5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	THAP domain	[U-99% 15N]	0,7 mM
10	Tris-HCl	natural abundance	50 mM
11	sodium chloride	natural abundance	30 mM
12	DTT	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 5.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 5.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 4.66 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2LAU, Strand ID: A Detail

Release date

2012-09-23

Citation

NMR studies of a new family of DNA binding proteins: the THAP proteins
Gervais, V., Campagne, S., Durand, J., Muller, I., Milon, A.
J. Biomol. NMR (2013), 56, 3-15, PubMed 23306615 , DOI 10.1007/s10858-012-9699-1 , Abstract

Related entities 1. THAP domain, : 1 : 6 : 52 entities Detail

Interaction partners 1. THAP domain, : 26 interactors Detail

More details for 26 interactors identified by 8 citations

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC