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BMRB: 17479

2L9N

Structure of the human Shwachman-Bodian-Diamond syndrome (SBDS) protein

Authors

Hilcenko, C., Freund, S.M.V., Warren, A.J.

Assembly

human SBDS

Entity

1. human SBDS (polymer, Thiol state: all free), 252 monomers, 28907.28 Da Detail

GSMSIFTPTN QIRLTNVAVV RMKRAGKRFE IACYKNKVVG WRSGVEKDLD EVLQTHSVFV NVSKGQVAKK EDLISAFGTD DQTEICKQIL TKGEVQVSDK ERHTQLEQMF RDIATIVADK CVNPETKRPY TVILIERAMK DIHYSVKTNK STKQQALEVI KQLKEKMKIE RAHMRLRFIL PVNEGKKLKE KLKPLIKVIE SEDYGQQLEI VCLIDPGCFR EIDELIKKET KGKGSLEVLN LKDVEEGDEK FE

Formula weight

28907.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 87.2 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.2 % (2672 of 3065)	87.6 % (1416 of 1617)	85.0 % (1003 of 1180)	94.4 % (253 of 268)
Backbone	95.8 % (1437 of 1500)	98.0 % (501 of 511)	93.9 % (698 of 743)	96.7 % (238 of 246)
Sidechain	80.6 % (1454 of 1804)	82.3 % (910 of 1106)	78.4 % (530 of 676)	63.6 % (14 of 22)
Aromatic	32.1 % (45 of 140)	57.1 % (40 of 70)	5.8 % (4 of 69)	100.0 % (1 of 1)
Methyl	94.7 % (284 of 300)	97.3 % (146 of 150)	92.0 % (138 of 150)

1. human SBDS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	150 mM
3	DTT	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 %
5	human_SBDS		0.48 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [15N]-labelled human SBDS for all mutants

#	Name	Isotope labeling	Concentration
15	potassium phosphate	natural abundance	25 mM
16	sodium chloride	natural abundance	150 mM
17	DTT	natural abundance	1 mM
18	sodium azide	natural abundance	0.05 %
19	human_SBDS		0.3 mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
22	potassium phosphate	natural abundance	25 mM
23	sodium chloride	natural abundance	150 mM
24	DTT	natural abundance	1 mM
25	sodium azide	natural abundance	0.05 %
26	human_SBDS		0.4 mM
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,13C]-labelled human WT SBDS

#	Name	Isotope labeling	Concentration
36	potassium phosphate	natural abundance	25 mM
37	sodium chloride	natural abundance	150 mM
38	DTT	natural abundance	1 mM
39	sodium azide	natural abundance	0.05 %
40	human_SBDS		0.4 mM
41	H2O	natural abundance	90 %
42	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L9N, Strand ID: A Detail

Release date

2011-05-04

Citation

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome
Finch, A.J., Hilcenko, C., Basse, N., Drynan, L.F., Goyenechea, B., Menne, T.F., Gonzalez Fernandez, A., Simpson, P., Arends, C.S., Donadieu, M.J., Bellanne-Chantelot, J., Costanzo, C., Boone, M., McKenzie, C., Freund, A.N., Warren, S.M.V.
Genes. Dev. (2011), 25, 917-929, PubMed 21536732 , DOI 10.1101/gad.623011 , Abstract

Entries sharing articles Swiss-Prot: 5 entries Detail

  Swiss-Prot: Q7Z2Z2 released on 2008-01-15
    Title ETUD1_HUMAN Entity Elongation factor Tu GTP-binding domain-containing protein 1
  Swiss-Prot: Q9Y3A5 released on 2000-05-30
    Title SBDS_HUMAN Entity Ribosome maturation protein SBDS
  Swiss-Prot: O55135 released on 1998-12-15
    Title IF6_MOUSE Entity Eukaryotic translation initiation factor 6
  Swiss-Prot: P56537 released on 1998-07-15
    Title IF6_HUMAN Entity Eukaryotic translation initiation factor 6
  Swiss-Prot: P70122 released on 1997-11-01
    Title SBDS_MOUSE Entity Ribosome maturation protein SBDS

Related entities 1. human SBDS, : 1 : 10 : 29 entities Detail

Interaction partners 1. human SBDS, : 10 interactors Detail

More details for 10 interactors identified by 5 citations

Experiments performed 21 experiments Detail

1 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

2 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

3 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

4 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

5 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

6 3D HNCACO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

7 3D HNCA

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

8 3D HNCO

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

9 3D HN(CO)CA

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

10 3D HBHA(CO)NH

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

11 3D HNCACB

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

12 3D HNCACO

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled yeast WT SBDS

#	Name	Isotope labeling	Concentration
29	potassium phosphate	natural abundance	25 mM
30	sodium chloride	natural abundance	150 mM
31	DTT	natural abundance	1 mM
32	sodium azide	natural abundance	0.05 %
33	human_SBDS		0.35 mM
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

13 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [15N]-labelled human SBDS for all mutants

#	Name	Isotope labeling	Concentration
15	potassium phosphate	natural abundance	25 mM
16	sodium chloride	natural abundance	150 mM
17	DTT	natural abundance	1 mM
18	sodium azide	natural abundance	0.05 %
19	human_SBDS		0.3 mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

14 3D 1H-15N NOESY

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	150 mM
3	DTT	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 %
5	human_SBDS		0.48 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

15 3D 1H-13C NOESY

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,13C]-labelled human WT SBDS

#	Name	Isotope labeling	Concentration
36	potassium phosphate	natural abundance	25 mM
37	sodium chloride	natural abundance	150 mM
38	DTT	natural abundance	1 mM
39	sodium azide	natural abundance	0.05 %
40	human_SBDS		0.4 mM
41	H2O	natural abundance	90 %
42	D2O	natural abundance	10 %

16 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,13C]-labelled human WT SBDS

#	Name	Isotope labeling	Concentration
36	potassium phosphate	natural abundance	25 mM
37	sodium chloride	natural abundance	150 mM
38	DTT	natural abundance	1 mM
39	sodium azide	natural abundance	0.05 %
40	human_SBDS		0.4 mM
41	H2O	natural abundance	90 %
42	D2O	natural abundance	10 %

17 3D HCCH-TOCSY

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,13C]-labelled human WT SBDS

#	Name	Isotope labeling	Concentration
36	potassium phosphate	natural abundance	25 mM
37	sodium chloride	natural abundance	150 mM
38	DTT	natural abundance	1 mM
39	sodium azide	natural abundance	0.05 %
40	human_SBDS		0.4 mM
41	H2O	natural abundance	90 %
42	D2O	natural abundance	10 %

18 3D HCCH-COSY

Instrument

Bruker DRX - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,13C]-labelled human WT SBDS

#	Name	Isotope labeling	Concentration
36	potassium phosphate	natural abundance	25 mM
37	sodium chloride	natural abundance	150 mM
38	DTT	natural abundance	1 mM
39	sodium azide	natural abundance	0.05 %
40	human_SBDS		0.4 mM
41	H2O	natural abundance	90 %
42	D2O	natural abundance	10 %

19 3D HN(CAN)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

20 3D (H)CC(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

21 3D H(CCCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details [2H,15N,13C]-labelled WT human SBDS

#	Name	Isotope labeling	Concentration
8	potassium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.05 %
12	human_SBDS		0.5 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_17479_2l9n.nef
Input source #2: Coordindates	2l9n.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-0--------10--------20--------30--------40--------50--------60--------70--------80--------90-------1
GSMSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSMSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDK
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

00-------110-------120-------130-------140-------150-------160-------170-------180-------190-------2
ERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIE
-------110-------120-------130-------140-------150-------160-------170-------180-------190-------200

00-------210-------220-------230-------240-------250
SEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE
||||||||||||||||||||||||||||||||||||||||||||||||||||
SEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE
-------210-------220-------230-------240-------250--

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	252	0	0	100.0

Content subtype: combined_17479_2l9n.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	2551		98.0 (chain: A, length: 252)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	6076 (1)	noe	98.0 (chain: A, length: 252)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 252, Sequence coverage: 98.0%

-0--------10--------20--------30--------40--------50--------60--------70--------80--------90-------1
GSMSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDK
   |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
...SIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDK

00-------110-------120-------130-------140-------150-------160-------170-------180-------190-------2
ERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIE
|||||  |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ERHTQ..QMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIE

00-------210-------220-------230-------240-------250
SEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE
||||||||||||||||||||||||||||||||||||||||||||||||||||
SEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE

Total number of assignments
- ¹H chemical shifts: 1350
- ¹³C chemical shifts: 953
- ¹⁵N chemical shifts: 248

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	THR	HG1	4.643
13	THR	HG1	4.638
31	CYS	HG	1.096
53	THR	HG1	4.641
77	THR	HG1	4.641
81	THR	HG1	4.643
89	THR	HG1	4.646
102	THR	HG1	4.645
113	THR	HG1	4.633
124	THR	HG1	4.905
146	THR	HG1	4.637
150	THR	HG1	4.346
228	THR	HG1	4.644

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1617	1337	82.7
¹³C chemical shifts	1180	953	80.8
¹⁵N chemical shifts	281	248	88.3

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	511	495	96.9
¹³C chemical shifts	504	457	90.7
¹⁵N chemical shifts	246	237	96.3

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1106	842	76.1
¹³C chemical shifts	676	496	73.4
¹⁵N chemical shifts	35	11	31.4

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	156	146	93.6
¹³C chemical shifts	156	138	88.5

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	38	54.3
¹³C chemical shifts	69	0	0.0
¹⁵N chemical shifts	1	1	100.0

Redox state of CYS
Peptide bond of PRO
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 252, Sequence coverage: 98.0%
- Total number of restraints: 6008
- Weight of restraints: 1.0 (6008)
- Potential type of restraints: square-well-parabolic (6008)
- Range of distance target values: 2.3, 4.15 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords human, NMR, protein, SBDS, structure

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Found 1 Document (1.94 seconds)

BMRB: 17479

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 5 entries Detail

Related entities 1. human SBDS, : 1 : 10 : 29 entities Detail

Interaction partners 1. human SBDS, : 10 interactors Detail

Experiments performed 21 experiments Detail

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