BMRB: 17489

1H, 13C, and 15N Chemical Shift Assignments for the FF domain L24A mutant

Authors

Korzhnev, D.M., Vernon, R.M., Religa, T.L., Hansen, A., Baker, D., Fersht, A.R., Kay, L.E.

Assembly

FF domain L24A mutant

Entity

1. FF domain L24A mutant (polymer, Thiol state: not present), 70 monomers, 8137.213 Da Detail

SQPAKKTYTW NTKEEAKQAF KEALKEKRVP SNASWEQAMK MIINDPRYSA LAKLSEKKQA FNAYKVQTEK

Formula weight

8137.213 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Monte Carlo

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 36.5 %, Completeness (bb): 76.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	36.5 % (319 of 874)	27.3 % (127 of 466)	37.9 % (125 of 330)	85.9 % (67 of 78)
Backbone	76.6 % (317 of 414)	91.2 % (125 of 137)	59.5 % (125 of 210)	100.0 % (67 of 67)
Sidechain	0.9 % (5 of 530)	0.6 % (2 of 329)	1.6 % (3 of 190)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 34)	0.0 % (0 of 32)	0.0 % (0 of 2)
Methyl	1.8 % (1 of 56)	0.0 % (0 of 28)	3.6 % (1 of 28)

1. FF Domain L24A

SQPAKKTYTW NTKEEAKQAF KEALKEKRVP SNASWEQAMK MIINDPRYSA LAKLSEKKQA FNAYKVQTEK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FF_Domain_L24A	[U-13C; U-15N; U-2H]		1.0 ~ 2.0 mM
2	sodium chloride	natural abundance		100 mM
3	sodium acetate	natural abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
4	FF_Domain_L24A	[U-100% 13C; U-100% 15N; U-50% 2H]		1.0 ~ 2.0 mM
5	sodium chloride	natural abundance		100 mM
6	sodium acetate	natural abundance		50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
7	FF_Domain_L24A	[U-13C; U-15N; U-2H]		1.0 ~ 2.0 mM
8	sodium chloride	natural abundance		100 mM
9	sodium acetate	natural abundance		50 mM
10	C12E5/n-hexanol	natural abundance		5 %

Hide sample codition

Protein Blocks Logo

Calculated from 9 models in PDB: 2L9V, Strand ID: A Detail

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RDC

45 RDC values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 303 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 303 K, pH 5.7

Experiment name CPMG

List #1 RDC_list_1, RDC code DNH, Field strength (¹H) 800 MHz

Release date

2011-06-06

Citation

Nonnative interactions in the FF domain folding pathway from an atomic resolution structure of a sparsely populated intermediate: an NMR relaxation dispersion study
Korzhnev, D.M., Vernon, R.M., Religa, T.L., Hansen, A.L., Baker, D., Fersht, A.R., Kay, L.E.
J. Am. Chem. Soc. (2011), 133, 10974-10982, PubMed 21639149 , DOI 10.1021/ja203686t , Abstract

Related entities 1. FF domain L24A mutant, : 1 : 7 : 31 entities Detail

Interaction partners 1. FF domain L24A mutant, : 96 interactors Detail

Experiments performed 2 experiments Detail