BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.9 % (1298 of 1460)	88.9 % (682 of 767)	88.1 % (495 of 562)	92.4 % (121 of 131)
Backbone	91.8 % (641 of 698)	91.9 % (217 of 236)	91.6 % (318 of 347)	92.2 % (106 of 115)
Sidechain	87.0 % (761 of 875)	87.6 % (465 of 531)	85.7 % (281 of 328)	93.8 % (15 of 16)
Aromatic	49.2 % (64 of 130)	50.8 % (33 of 65)	46.9 % (30 of 64)	100.0 % (1 of 1)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. L4W

MFLERNEVKR LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS DFNGAIIIFR DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH WHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
11	L4W	[U-99% 13C; U-99% 15N]	600 uM
12	potassium phosphate pH 6	natural abundance	18 mM
13	potassium chloride	natural abundance	45 mM
14	DSS	natural abundance	1 uM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
17	L4W	natural abundance	600 uM
18	potassium phosphate pH 6	[U-99% 2H]	18 mM
19	potassium chloride	[U-99% 2H]	45 mM
20	D2O	natural abundance	100 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
21	L4W	[U-99% 13C; U-99% 15N]	600 uM
22	potassium phosphate pH 6	natural abundance	18 mM
23	potassium chloride	natural abundance	45 mM
24	DTT	natural abundance	0.9 mM
25	DMPC/DHPC q=3	natural abundance	6.5 %
26	CTAB	natural abundance	0.67 mg/mL
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L9W, Strand ID: A Detail

Release date

2011-06-21

Citation

A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop
Martin-Tumasz, S., Richie, A.C., Clos, L.J., Brow, D.A., Butcher, S.E.
Nucleic Acids Res. (2011), 39, 7837-7847, PubMed 21653550 , DOI 10.1093/nar/gkr455 , Abstract

Related entities 1. C-terminal domain of Prp24, : 1 : 4 : 2 entities Detail

Interaction partners 1. C-terminal domain of Prp24, : 18 interactors Detail

More details for 18 interactors identified by 8 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

3 3D 1H-15N NOESY

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

6 3D HBHA(CO)NH

Instrument

Varian Uniform NMR System - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Varian Uniform NMR System - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

8 3D H(CCO)NH

Instrument

Varian Uniform NMR System - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

9 2D 1H-13C HSQC aliphatic

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

10 2D 1H-13C HSQC aromatic

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

11 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
17	L4W	natural abundance	600 uM
18	potassium phosphate pH 6	[U-99% 2H]	18 mM
19	potassium chloride	[U-99% 2H]	45 mM
20	D2O	natural abundance	100 %

12 3D HCCH-TOCSY

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

13 3D 1H-13C NOESY aliphatic

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

14 3D 1H-13C NOESY aromatic

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
7	L4W	[U-99% 13C; U-99% 15N]	600 uM
8	potassium phosphate pH 6	[U-99% 2H]	18 mM
9	potassium chloride	[U-99% 2H]	45 mM
10	D2O	natural abundance	100 %

15 2D 1H-13C HSQC aliphatic

Instrument

Varian Uniform NMR System - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
11	L4W	[U-99% 13C; U-99% 15N]	600 uM
12	potassium phosphate pH 6	natural abundance	18 mM
13	potassium chloride	natural abundance	45 mM
14	DSS	natural abundance	1 uM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

16 2D-NHSQC-IPAP

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

17 2D-NHSQC-IPAP

Instrument

Bruker DMX - 750 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
21	L4W	[U-99% 13C; U-99% 15N]	600 uM
22	potassium phosphate pH 6	natural abundance	18 mM
23	potassium chloride	natural abundance	45 mM
24	DTT	natural abundance	0.9 mM
25	DMPC/DHPC q=3	natural abundance	6.5 %
26	CTAB	natural abundance	0.67 mg/mL
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

18 3D-Jmodulated-CHSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	L4W	[U-99% 13C; U-99% 15N]	600 uM
2	potassium phosphate pH 6	natural abundance	18 mM
3	potassium chloride	natural abundance	45 mM
4	DTT	natural abundance	0.9 mM
5	H2O	natural abundance	9 %
6	D2O	natural abundance	10 %

19 3D-Jmodulated-CHSQC

Instrument

Bruker DMX - 750 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
21	L4W	[U-99% 13C; U-99% 15N]	600 uM
22	potassium phosphate pH 6	natural abundance	18 mM
23	potassium chloride	natural abundance	45 mM
24	DTT	natural abundance	0.9 mM
25	DMPC/DHPC q=3	natural abundance	6.5 %
26	CTAB	natural abundance	0.67 mg/mL
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_17490_2l9w.nef
Input source #2: Coordindates	2l9w.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-------300-------310-------320-------330-------340-------350-------360-------370-------380-------390
MFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDM
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDM
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-------400-------
KRYYNNQQNHWHHHHHH
|||||||||||||||||
KRYYNNQQNHWHHHHHH
-------110-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	117	0	0	100.0

Content subtype: combined_17490_2l9w.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1292		94.0 (chain: A, length: 117)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	1886 (1)	noe	92.3 (chain: A, length: 117)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	53 (1)	hbond	49.6 (chain: A, length: 117)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	177 (177)	.	90.6 (chain: A, length: 117)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	120 (120)	.	77.8 (chain: A, length: 117)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 94.0%
- Total number of assignments
  - ¹H chemical shifts: 679
  - ¹³C chemical shifts: 492
  - ¹⁵N chemical shifts: 121
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	767	679	88.5
¹³C chemical shifts	562	492	87.5
¹⁵N chemical shifts	138	121	87.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	236	218	92.4
¹³C chemical shifts	234	214	91.5
¹⁵N chemical shifts	115	106	92.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	531	461	86.8
¹³C chemical shifts	328	278	84.8
¹⁵N chemical shifts	23	15	65.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	64	94.1
¹³C chemical shifts	68	63	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	33	50.8
¹³C chemical shifts	64	30	46.9
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 92.3%
- Total number of restraints: 1875
- Weight of restraints: 1.0 (1875)
- Potential type of restraints: square-well-parabolic (1875)
- Range of distance target values: 2.35, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 49.6%
  - Total number of restraints: 53
  - Weight of restraints: 1.0 (53)
  - Potential type of restraints: square-well-parabolic (53)
  - Range of distance target values: 1.95, 3.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 90.6%
- Total number of restraints: 177
- Total number of restraints per polymer type: 177
- Weight of restraints: 1.0 (177)
- Potential type of restraints: square-well-parabolic (177)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 77.8%

-------300-------310-------320-------330-------340-------350-------360-------370-------380-------390
MFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDM
 |||||||||||||| |||||||||  | | |||| |||| ||||| |||| |||||||||| | |||||||||||||| |||||| |||| |||||| |
.FLERNEVKRLLASR.SKELETLIC..P.S.KVSP.LICQ.LQEEI.INEK.IRKILLVSDF.G.IIIFRDSKFAAKML.ILNGSQ.QGKV.RSGTIN.M
-------300-------310-------320-------330-------340-------350-------360-------370-------380-------390

-------400-------
KRYYNNQQNHWHHHHHH
| | |||||        
K.Y.NNQQN        
---------

Total number of restraints: 120
Potential type of restraints: undefined (120)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords Prp24, RRM, Splicing, U6 snRNP

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Found 1 Document (1.613 seconds)

BMRB: 17490

Sample #1

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Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. C-terminal domain of Prp24, : 1 : 4 : 2 entities Detail

Interaction partners 1. C-terminal domain of Prp24, : 18 interactors Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 6 contents Detail