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BMRB: 17493

2L9Y

Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)

Authors

Koharudin, L.M.I., Viscomi, A.R., Montanini, B., Kershaw, M.J., Talbot, N.J., Ottonello, S., Gronenborn, A.M.

Assembly

MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein MGG 03307

Entity

1. MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein MGG 03307 (polymer, Thiol state: all free), 167 monomers, 18152.64 Da Detail

GNYAGNFSGS SRDICLDGAR LRAECRRGDG GYSTSVIDLN RYLSNDNGHF RWVSGGGGGG GTATVTVQQG DTLRDIGRRF DCDFHEIARR NNIQNEDLIY PGQVLQVPTK GGSGGGAGNF WDSARDVRLV DGGKVLEAEL RYSGGWNRSR IYLDEHIGNR NGELIHC

Formula weight

18152.64 Da

Source organism

Pyricularia oryzae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.1 %, Completeness (bb): 82.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.1 % (1382 of 1816)	83.2 % (785 of 943)	62.4 % (429 of 688)	90.8 % (168 of 185)
Backbone	82.3 % (821 of 998)	95.0 % (344 of 362)	66.5 % (313 of 471)	99.4 % (164 of 165)
Sidechain	73.1 % (698 of 955)	75.9 % (441 of 581)	71.5 % (253 of 354)	20.0 % (4 of 20)
Aromatic	28.0 % (42 of 150)	52.0 % (39 of 75)	0.0 % (0 of 72)	100.0 % (3 of 3)
Methyl	95.9 % (142 of 148)	94.6 % (70 of 74)	97.3 % (72 of 74)

1. entity

GNYAGNFSGS SRDICLDGAR LRAECRRGDG GYSTSVIDLN RYLSNDNGHF RWVSGGGGGG GTATVTVQQG DTLRDIGRRF DCDFHEIARR NNIQNEDLIY PGQVLQVPTK GGSGGGAGNF WDSARDVRLV DGGKVLEAEL RYSGGWNRSR IYLDEHIGNR NGELIHC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details 15N-labeled MoCVNH-LysM

#	Name	Isotope labeling	Concentration
1	MoCVNH-LysM	[U-100% 15N]	0.5 (±0.01) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium acetate	natural abundance	25.0 ~ 50.0 mM
5	NaCl	natural abundance	20 mM
6	NaN3	natural abundance	3 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details 13C,15N-labeled sample

#	Name	Isotope labeling	Concentration
7	MoCVNH-LysM	[U-100% 13C; U-100% 15N]	0.5 (±0.01) mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	sodium acetate	natural abundance	25.0 ~ 50.0 mM
11	NaCl	natural abundance	20 mM
12	NaN3	natural abundance	3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.101329

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.101329

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.101329

Referencing offset: -0.18 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2L9Y, Strand ID: A Detail

Release date

2011-05-05

Citation

Structure-function analysis of a CVNH-LysM lectin expressed during plant infection by the rice blast fungus Magnaporthe oryzae
Koharudin, L.M.I., Viscomi, A.R., Montanini, B., Kershaw, M.J., Talbot, N.J., Ottonello, S., Gronenborn, A.M.
Structure (2011), 19, 662-674, PubMed 21565701 , DOI 10.1016/j.str.2011.03.004 , Abstract

Related entities 1. MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein MGG 03307, : 1 : 4 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz 3D data collection for backbone and side chain assignments

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details 15N-labeled MoCVNH-LysM

#	Name	Isotope labeling	Concentration
1	MoCVNH-LysM	[U-100% 15N]	0.5 (±0.01) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium acetate	natural abundance	25.0 ~ 50.0 mM
5	NaCl	natural abundance	20 mM
6	NaN3	natural abundance	3 mM

2 3D HNCACB