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BMRB: 17518

2LAA

Solution Strucuture of CBM25-1 of beta/alpha-amylase from Paenibacillus polymyxa

Authors

Horibe, I., Takahashi, R., Yoshida, T., Ohkubo, T., Sumitani, J., Nishimura, S.

Assembly

Starch Binding Domain 1 of beta/alpha-amylase

Entity

1. Starch Binding Domain 1 of beta/alpha-amylase (polymer), 104 monomers, 10737.44 Da Detail

GGTGNKVTIY YKKGFNSPYI HYRPAGGSWT AAPGVKMQDA EISGYAKITV DIGSASQLEA AFNDGNNNWD SNNTKNYSFS TGTSTYTPGN SGNAGTITSG APAG

Formula weight

10737.44 Da

Source organism

Paenibacillus polymyxa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.6 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (1021 of 1102)	91.3 % (514 of 563)	92.9 % (395 of 425)	98.2 % (112 of 114)
Backbone	97.7 % (600 of 614)	96.3 % (211 of 219)	98.6 % (292 of 296)	98.0 % (97 of 99)
Sidechain	88.4 % (509 of 576)	88.1 % (303 of 344)	88.0 % (191 of 217)	100.0 % (15 of 15)
Aromatic	57.0 % (65 of 114)	57.9 % (33 of 57)	54.5 % (30 of 55)	100.0 % (2 of 2)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. entity

GGTGNKVTIY YKKGFNSPYI HYRPAGGSWT AAPGVKMQDA EISGYAKITV DIGSASQLEA AFNDGNNNWD SNNTKNYSFS TGTSTYTPGN SGNAGTITSG APAG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 15N]		0.5-1.2 mM
2	sodium acetate	[U-99% 13C; U-99% 15N]		0.5-1.2 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
3	sodium acetate	[U-100% 15N]		0.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LAA, Strand ID: A Detail

Release date

2011-04-03

Citation

A functional and structural analysis of tundem family 25 carbohydrate-binding modules from Paenibacillus polymyxa beta/alpha-amylase
Takahashi, R., Horibe, I., Fukada, H., Yoshida, T., Ohkubo, T., Inui, T., Nishimura, S., Sumitani, J.
Protein Sci.

Related entities 1. Starch Binding Domain 1 of beta/alpha-amylase, : 1 : 1 : 9 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC