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BMRB: 17519

2LAB

Solution Strucuture of CBM25-2 of beta/alpha-amylase from Paenibacillus polymyxa

Authors

Takahashi, R., Yoshida, T., Ohkubo, T., Sumitani, J., Nishimura, S.

Assembly

Starch Binding Domain 2 of beta/alpha-amylase

Entity

1. Starch Binding Domain 2 of beta/alpha-amylase (polymer), 104 monomers, 10835.62 Da Detail

GGTTNKVTVY YKKGFNSPYI HYRPAGGSWT AAPGVKMQDA EISGYAKITV DIGSASQLEA AFNDGNNNWD SNNTKNYLFS TGTSTYTPGS NGAAGTIRTG APSG

Formula weight

10835.62 Da

Source organism

Paenibacillus polymyxa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 89.2 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (992 of 1112)	86.3 % (490 of 568)	91.2 % (393 of 431)	96.5 % (109 of 113)
Backbone	95.0 % (583 of 614)	93.1 % (203 of 218)	95.6 % (284 of 297)	97.0 % (96 of 99)
Sidechain	84.5 % (496 of 587)	82.0 % (287 of 350)	87.9 % (196 of 223)	92.9 % (13 of 14)
Aromatic	61.4 % (70 of 114)	61.4 % (35 of 57)	60.0 % (33 of 55)	100.0 % (2 of 2)
Methyl	96.7 % (87 of 90)	97.8 % (44 of 45)	95.6 % (43 of 45)

1. carbohydrate binding module

GGTTNKVTVY YKKGFNSPYI HYRPAGGSWT AAPGVKMQDA EISGYAKITV DIGSASQLEA AFNDGNNNWD SNNTKNYLFS TGTSTYTPGS NGAAGTIRTG APSG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 15N]		0.5-1.2 mM
2	sodium acetate	[U-13C; U-15N]		0.5-1.2 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
3	sodium acetate	[U-100% 15N]		0.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP	methyl protons	internal	indirect	0.1013291
¹H	TSP	methyl protons	internal	direct	1.0
¹³N	TSP	methyl protons	internal	indirect	0.2514495

Referencing offset: 0.42 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LAB, Strand ID: A Detail

Release date

2012-08-06

Citation

A functional and structural analysis of tundem family 25 carbohydrate-binding modules from Paenibacillus polymyxa beta/alpha-amylase
Takahashi, R., Horibe, I., Fukada, H., Yoshida, T., Ohkubo, T., Inui, T., Nishimura, S., Sumitani, J.
Protein Sci.

Related entities 1. Starch Binding Domain 2 of beta/alpha-amylase, : 1 : 1 : 9 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC