BMRBj - BMREntryMaster

Found 1 Document (1.218 seconds)

BMRB: 17521

2LAE

Solution structure of an E. coli lipoprotein

Authors

Pardi, A., Warner, L.

Assembly

BamC

Entity

1. BamC (polymer, Thiol state: not present), 249 monomers, 26975.73 Da Detail

GAMGDTASLL VENGRGNTLW PQVVSVLQAK NYTITQRDDA GQTLTTDWVQ WNRLDEDEQY RGRYQISVKP QGYQQAVTVK LLNLEQAGKP VADAASMQRY STEMMNVISA GLDKSATDAA NAAQNRASTT MDVQSAADDT GLPMLVVRGP FNVVWQRLPA ALEKVGMKVT DSTRSQGNMA VTYKPLSDSD WQELGASDPG LASGDYKLQV GDLDNRSSLQ FIDPKGHTLT QSQNDALVAV FQAAFSKPG

Formula weight

26975.73 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.9 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (2295 of 2769)	77.3 % (1106 of 1430)	88.6 % (940 of 1061)	89.6 % (249 of 278)
Backbone	98.4 % (1451 of 1474)	97.6 % (495 of 507)	98.5 % (717 of 728)	100.0 % (239 of 239)
Sidechain	70.1 % (1069 of 1525)	66.2 % (611 of 923)	79.6 % (448 of 563)	25.6 % (10 of 39)
Aromatic	9.4 % (15 of 160)	12.5 % (10 of 80)	0.0 % (0 of 75)	100.0 % (5 of 5)
Methyl	93.2 % (259 of 278)	93.5 % (130 of 139)	92.8 % (129 of 139)

1. BamC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
9	BamC	natural abundance	1.1 mM
10	sodium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	50 mM
12	EDTA	natural abundance	0.1 mM
13	sodium azide	natural abundance	0.02 %
14	HALT	natural abundance	1 na
15	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
16	BamC	natural abundance	1.1 mM
17	sodium phosphate	natural abundance	50 mM
18	sodium chloride	natural abundance	50 mM
19	EDTA	natural abundance	0.1 mM
20	sodium azide	natural abundance	0.02 %
21	HALT	natural abundance	1 na
22	Pf1 phage	natural abundance	21 mg/mL
23	H2O	natural abundance	90 %
24	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	TSP	methyl protons	-0.012 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	TSP	methyl protons	-0.012 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	TSP	methyl protons	-0.012 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	TSP	methyl protons	-0.012 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 9 models in PDB: 2LAE, Strand ID: A Detail

RDC

156 RDC values in 1 lists
Field strength (¹H) 599.71 MHz, Pressure 1 atm, Temperature 273 K, pH 6.6 Detail

Release date

2011-05-31

Citation

Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set
Warner, L.R., Varga, K., Lange, O.F., Baker, S.L., Baker, D., Sousa, M.C., Pardi, A.
J. Mol. Biol. (2011), 411, 83-95, PubMed 21624375 , DOI 10.1016/j.jmb.2011.05.022 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P0A903 released on 2005-07-19
Title BAMC_ECOLI Entity Outer membrane protein assembly factor BamC

Related entities 1. BamC, : 1 : 1 : 6 : 13 entities Detail

Interaction partners 1. BamC, : 8 interactors Detail

More details for 8 interactors identified by 6 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian VNMRS - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 3D 1H-13C NOESY

Instrument

Varian VNMRS - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
9	BamC	natural abundance	1.1 mM
10	sodium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	50 mM
12	EDTA	natural abundance	0.1 mM
13	sodium azide	natural abundance	0.02 %
14	HALT	natural abundance	1 na
15	D2O	natural abundance	100 %

3 3D 1H-15N NOESY

Instrument

Varian VNMRS - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D CBCA(CO)NH

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D HNHAHB

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D H(CCO)NH

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

9 3D HBHA(CO)NH

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

11 3D (H)C(CO)NH

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

12 3D HN(CA)CO

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

13 2D 1H-15N HSQC IPAP

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
16	BamC	natural abundance	1.1 mM
17	sodium phosphate	natural abundance	50 mM
18	sodium chloride	natural abundance	50 mM
19	EDTA	natural abundance	0.1 mM
20	sodium azide	natural abundance	0.02 %
21	HALT	natural abundance	1 na
22	Pf1 phage	natural abundance	21 mg/mL
23	H2O	natural abundance	90 %
24	D2O	natural abundance	10 %

14 2D 1H-15N HSQC IPAP

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	BamC	natural abundance	1.1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	0.1 mM
5	sodium azide	natural abundance	0.02 %
6	HALT	natural abundance	1 na
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

nullKeywords lipoprotein, Rosetta

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Found 1 Document (1.218 seconds)

BMRB: 17521

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. BamC, : 1 : 1 : 6 : 13 entities Detail

Interaction partners 1. BamC, : 8 interactors Detail

Experiments performed 14 experiments Detail

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