Glycyl-tRNA(GCC) anticodon stem-loop from Bacillus subtilis
Polymer type: polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 75.0 % (198 of 264) | 71.5 % (108 of 151) | 79.6 % (90 of 113) |
Suger, PO4 | 77.0 % (144 of 187) | 79.4 % (81 of 102) | 74.1 % (63 of 85) |
Nucleobase | 70.1 % (54 of 77) | 55.1 % (27 of 49) | 96.4 % (27 of 28) |
Aromatic | 83.1 % (54 of 65) | 73.0 % (27 of 37) | 96.4 % (27 of 28) |
1. glycyl-tRNA
GGGCCUUGCC AAGGUCCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA | [U-98% 13C; U-98% 15N] | 0.02 ~ 0.04 mM | |
2 | D2O | natural abundance | 100 % | |
3 | KH2PO4 | natural abundance | 10 mM | |
4 | K2HPO4 | natural abundance | 10 mM | |
5 | KCl | natural abundance | 10 mM | |
6 | EDTA | natural abundance | 0.2 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA | natural abundance | 0.02 ~ 0.04 mM | |
8 | D2O | natural abundance | 100 % | |
9 | KH2PO4 | natural abundance | 10 mM | |
10 | K2HPO4 | natural abundance | 10 mM | |
11 | KCl | natural abundance | 10 mM | |
12 | EDTA | natural abundance | 0.2 mM |