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BMRB: 17576

2M3T

1H, 13C, and 15N assignments of wild-type gamma-S crystallin

Authors

Brubaker, W.D., Martin, R.W.

Assembly

gamma-S-WT

Entity

1. gamma-S-WT (polymer, Thiol state: all free), 178 monomers, 20932.39 Da Detail

GSKTGTKITF YEDKNFQGRR YDCDCDCADF HTYLSRCNSI KVEGGTWAVY ERPNFAGYMY ILPQGEYPEY QRWMGLNDRL SSCRAVHLPS GGQYKIQIFE KGDFSGQMYE TTEDCPSIME QFHMREIHSC KVLEGVWIFY ELPNYRGRQY LLDKKEYRKP IDWGAASPAV QSFRRIVE

Formula weight

20932.39 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 87.8 %, Completeness (bb): 92.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.8 % (1882 of 2144)	87.4 % (983 of 1125)	87.6 % (728 of 831)	91.0 % (171 of 188)
Backbone	92.2 % (970 of 1052)	92.8 % (337 of 363)	92.5 % (480 of 519)	90.0 % (153 of 170)
Sidechain	84.4 % (1059 of 1255)	84.0 % (640 of 762)	84.4 % (401 of 475)	100.0 % (18 of 18)
Aromatic	52.3 % (139 of 266)	52.6 % (70 of 133)	50.4 % (65 of 129)	100.0 % (4 of 4)
Methyl	95.5 % (126 of 132)	93.9 % (62 of 66)	97.0 % (64 of 66)

1. gamma-S-WT

GSKTGTKITF YEDKNFQGRR YDCDCDCADF HTYLSRCNSI KVEGGTWAVY ERPNFAGYMY ILPQGEYPEY QRWMGLNDRL SSCRAVHLPS GGQYKIQIFE KGDFSGQMYE TTEDCPSIME QFHMREIHSC KVLEGVWIFY ELPNYRGRQY LLDKKEYRKP IDWGAASPAV QSFRRIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 4.5, Details pH 4.5 acetate buffer

#	Name	Isotope labeling	Concentration
1	gamma-S-WT	[U-100% 13C; U-100% 15N]	2.11 mM
2	acetic acid	natural abundance	10 mM
3	sodium azide	natural abundance	0.05 %
4	D2O	[U-2H]	10 %
5	TSP	[2,2,3,3-d4]	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: 0.8 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 2M3T, Strand ID: A Detail

Release date

2011-05-11

Citation

Separating instability from aggregation propensity in γS-crystallin variants
Brubaker, W.D., Freites, J., Golchert, K.J., Shapiro, R.A., Morikis, V., Tobias, D.J., Martin, R.W.
Biophys. J. (2011), 100, 498-506, PubMed 21244846 , DOI 10.1016/j.bpj.2010.12.3691 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17582 released on 2011-05-11
Title 1H, 13C, and 15N assignments of cataract-related variant gamma-S-G18V crystallin

Related entities 1. gamma-S-WT, : 1 : 1 : 6 : 5 : 259 entities Detail

Interaction partners 1. gamma-S-WT, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC