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BMRB: 17582

2M3U

1H, 13C, and 15N assignments of cataract-related variant gamma-S-G18V crystallin

Authors

Brubaker, W.D., Martin, R.W.

Assembly

gamma-S-G18V

Entity

1. gamma-S-G18V (polymer, Thiol state: all free), 178 monomers, 20974.47 Da Detail

GSKTGTKITF YEDKNFQVRR YDCDCDCADF HTYLSRCNSI KVEGGTWAVY ERPNFAGYMY ILPQGEYPEY QRWMGLNDRL SSCRAVHLPS GGQYKIQIFE KGDFSGQMYE TTEDCPSIME QFHMREIHSC KVLEGVWIFY ELPNYRGRQY LLDKKEYRKP IDWGAASPAV QSFRRIVE

Formula weight

20974.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.2 %, Completeness: 85.2 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.2 % (1830 of 2149)	84.7 % (955 of 1127)	84.8 % (707 of 834)	89.4 % (168 of 188)
Backbone	91.1 % (958 of 1052)	92.3 % (334 of 362)	91.0 % (473 of 520)	88.8 % (151 of 170)
Sidechain	81.6 % (1029 of 1261)	81.2 % (621 of 765)	81.8 % (391 of 478)	94.4 % (17 of 18)
Aromatic	55.3 % (147 of 266)	57.1 % (76 of 133)	51.9 % (67 of 129)	100.0 % (4 of 4)
Methyl	94.9 % (129 of 136)	95.6 % (65 of 68)	94.1 % (64 of 68)

1. gamma-S-G18V

GSKTGTKITF YEDKNFQVRR YDCDCDCADF HTYLSRCNSI KVEGGTWAVY ERPNFAGYMY ILPQGEYPEY QRWMGLNDRL SSCRAVHLPS GGQYKIQIFE KGDFSGQMYE TTEDCPSIME QFHMREIHSC KVLEGVWIFY ELPNYRGRQY LLDKKEYRKP IDWGAASPAV QSFRRIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 4.5, Details pH 4.5 acetate buffer

#	Name	Isotope labeling	Concentration
1	gamma-S-G18V	[U-100% 13C; U-100% 15N]	1.50 mM
2	acetic acid	natural abundance	10 mM
3	sodium azide	natural abundance	0.05 %
4	D2O	[U-2H]	10 %
5	TSP	[2,2,3,3-d4]	2 mM

Chem. Shift Complete2

Sequence coverage: 24.7 %, Completeness: 49.0 %, Completeness (bb): 54.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	49.0 % (2104 of 4298)	48.3 % (1088 of 2254)	48.4 % (807 of 1668)	55.6 % (209 of 376)
Backbone	54.7 % (1151 of 2104)	55.5 % (402 of 724)	53.8 % (559 of 1040)	55.9 % (190 of 340)
Sidechain	45.1 % (1137 of 2522)	44.9 % (687 of 1530)	45.1 % (431 of 956)	52.8 % (19 of 36)
Aromatic	31.6 % (168 of 532)	33.5 % (89 of 266)	29.1 % (75 of 258)	50.0 % (4 of 8)
Methyl	48.9 % (133 of 272)	49.3 % (67 of 136)	48.5 % (66 of 136)

1. gamma-S-G18V

GSKTGTKITF YEDKNFQVRR YDCDCDCADF HTYLSRCNSI KVEGGTWAVY ERPNFAGYMY ILPQGEYPEY QRWMGLNDRL SSCRAVHLPS GGQYKIQIFE KGDFSGQMYE TTEDCPSIME QFHMREIHSC KVLEGVWIFY ELPNYRGRQY LLDKKEYRKP IDWGAASPAV QSFRRIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 4.5, Details pH 4.5 acetate buffer

#	Name	Isotope labeling	Concentration
1	gamma-S-G18V	[U-100% 13C; U-100% 15N]	1.50 mM
2	acetic acid	natural abundance	10 mM
3	sodium azide	natural abundance	0.05 %
4	D2O	[U-2H]	10 %
5	TSP	[2,2,3,3-d4]	2 mM

Protein Blocks Logo

Calculated from 21 models in PDB: 2M3U, Strand ID: A Detail

Release date

2011-05-11

Citation

Separating instability from aggregation propensity in γS-crystallin variants
Brubaker, W.D., Freites, J., Golchert, K.J., Shapiro, R.A., Morikis, V., Tobias, D.J., Martin, R.W.
Biophys. J. (2011), 100, 498-506, PubMed 21244846 , DOI 10.1016/j.bpj.2010.12.3691 , Abstract

Related entities 1. gamma-S-G18V, : 1 : 6 : 256 entities Detail

Interaction partners 1. gamma-S-G18V, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC