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BMRB: 17594

2LC3

Solution NMR structure of a helical bundle domain from human E3 ligase HECTD1. Northeast structural genomics consortium (NESG) target HT6305A

Authors

Lemak, A., Yee, A., Houliston, S., Garcia, M., Arrowsmith, C., Dhe-Paganon, S.

Assembly

hs00411

Entity

1. hs00411 (polymer, Thiol state: all free), 88 monomers, 10062.22 Da Detail

MKDSDKEKEN GKMGCWSIEH VEQYLGTDEL PKNDLITYLQ KNADAAFLRH WKLTGTNKSI RKNRNCSQLI AAYKDFCEHG TKSGLNQG

Formula weight

10062.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (968 of 1042)	92.7 % (509 of 549)	92.4 % (363 of 393)	96.0 % (96 of 100)
Backbone	97.9 % (515 of 526)	98.4 % (179 of 182)	98.1 % (252 of 257)	96.6 % (84 of 87)
Sidechain	89.4 % (534 of 597)	89.9 % (330 of 367)	88.5 % (192 of 217)	92.3 % (12 of 13)
Aromatic	87.5 % (70 of 80)	87.5 % (35 of 40)	86.8 % (33 of 38)	100.0 % (2 of 2)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. entity

MKDSDKEKEN GKMGCWSIEH VEQYLGTDEL PKNDLITYLQ KNADAAFLRH WKLTGTNKSI RKNRNCSQLI AAYKDFCEHG TKSGLNQG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.7

#	Name	Isotope labeling	Concentration
1	hs00411	[U-13C; U-15N]	0.5 mM
2	TRIS	natural abundance	10 mM
3	sodium chloride	natural abundance	500 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LC3, Strand ID: A Detail

Release date

2012-08-06

Citation

NMR solution structure of a helical bundle from the E3 ligase HECTD1
Lemak, A., Yee, A., Houliston, S., Garcia, M., Arrowsmith, C., Dhe-Paganon, S.
Not known

Related entities 1. hs00411, : 1 : 4 : 1 : 1 entities Detail

Interaction partners 1. hs00411, : 20 interactors Detail

More details for 20 interactors identified by 16 citations

Experiments performed 9 experiments Detail

1 3D HNCO