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BMRB: 17608

2MBG

RLIP76 (GAP-GBD)

Authors

Rajasekar, K.V., Campbell, L.J., Nietlispach, D., Owen, D., Mott, H.R.

Assembly

RLIP76-GAP-GBD

Entity

1. RLIP76-GAP-GBD (polymer, Thiol state: all free), 265 monomers, 30972.74 Da Detail

HMPNLKPIFG IPLADAVERT MMYDGIRLPA VFRECIDYVE KYGMKCEGIY RVSGIKSKVD ELKAAYDREE STNLEDYEPN TVASLLKQYL RDLPENLLTK ELMPRFEEAC GRTTETEKVQ EFQRLLKELP ECNYLLISWL IVHMDHVIAK ELETKMNIQN ISIVLSPTVQ ISNRVLYVFF THVQELFGNV VLKQVMKPLR WSNMATMPTL PETQAGIKEE IRRQEFLLNC LHRDLQGGIK DLSKEERLWE VQRILTALKR KLREA

Formula weight

30972.74 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.8 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.8 % (2953 of 3251)	90.3 % (1542 of 1707)	89.8 % (1137 of 1266)	98.6 % (274 of 278)
Backbone	95.2 % (1491 of 1566)	95.8 % (506 of 528)	93.6 % (735 of 785)	98.8 % (250 of 253)
Sidechain	88.0 % (1708 of 1940)	88.0 % (1037 of 1179)	87.9 % (647 of 736)	96.0 % (24 of 25)
Aromatic	84.6 % (176 of 208)	91.3 % (95 of 104)	77.2 % (78 of 101)	100.0 % (3 of 3)
Methyl	98.5 % (323 of 328)	98.8 % (162 of 164)	98.2 % (161 of 164)

1. RLIP76-GAP-GBD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.9

#	Name	Isotope labeling	Concentration
1	RLIP76-GAP-GBD	natural abundance	0.7 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	DTT	natural abundance	5 mM
6	H20	natural abundance	90 %
7	D20	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.9

#	Name	Isotope labeling	Concentration
8	RLIP76-GAP-GBD	[U-100% 15N]	0.7 mM
9	TRIS	natural abundance	20 mM
10	sodium chloride	natural abundance	100 mM
11	sodium azide	natural abundance	0.05 %
12	DTT	natural abundance	5 mM
13	H20	natural abundance	90 %
14	D20	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.9

#	Name	Isotope labeling	Concentration
15	RLIP76-GAP-GBD	[U-100% 13C; U-100% 15N]	0.6 mM
16	TRIS	natural abundance	20 mM
17	sodium chloride	natural abundance	100 mM
18	sodium azide	natural abundance	0.05 %
19	DTT	natural abundance	5 mM
20	H20	natural abundance	90 %
21	D20	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.9

#	Name	Isotope labeling	Concentration
22	RLIP76-GAP-GBD	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
23	TRIS	natural abundance	20 mM
24	sodium chloride	natural abundance	100 mM
25	sodium azide	natural abundance	0.05 %
26	DTT	natural abundance	5 mM
27	H20	natural abundance	90 %
28	D20	natural abundance	10 %

Protein Blocks Logo

Calculated from 35 models in PDB: 2MBG, Strand ID: A Detail

Release date

2011-10-11

Citation

1H, 13C and 15N resonance assignments of the GTPase-activating (GAP) and Ral binding domains (GBD) of RLIP76 (RalBP1)
Rajasekar, K.V., Campbell, L.J., Nietlispach, D., Owen, D., Mott, H.R.
Biomol. NMR Assign. (2012), 6, 119-122, PubMed 21915608 , DOI 10.1007/s12104-011-9337-y , Abstract

Related entities 1. RLIP76-GAP-GBD, : 1 : 19 entities Detail

Interaction partners 1. RLIP76-GAP-GBD, : 37 interactors Detail

More details for 37 interactors identified by 13 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N-TROSY