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BMRB: 17609

2LCE

Chemical shift assignment of Hr4436B from Homo Sapiens, Northeast

Authors

Lee, H., Shastry, R., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Prestegard, J.H.

Assembly

Hr4436B

Entity

1. Hr4436B (polymer), 74 monomers, 8615.585 Da Detail

MGHHHHHHSH MTHSDKPYKC DRCQASFRYK GNLASHKTVH TGEKPYRCNI CGAQFNRPAN LKTHTRIHSG EKPY

2. ZN (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

8746.403 Da

Max. entity weight

8615.585 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.4 %, Completeness: 71.3 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.3 % (616 of 864)	68.8 % (313 of 455)	73.0 % (243 of 333)	78.9 % (60 of 76)
Backbone	80.0 % (349 of 436)	77.2 % (115 of 149)	81.6 % (177 of 217)	81.4 % (57 of 70)
Sidechain	65.2 % (324 of 497)	64.7 % (198 of 306)	66.5 % (123 of 185)	50.0 % (3 of 6)
Aromatic	39.0 % (39 of 100)	40.0 % (20 of 50)	38.0 % (19 of 50)
Methyl	78.9 % (30 of 38)	78.9 % (15 of 19)	78.9 % (15 of 19)

1. Hr4436B

MGHHHHHHSH MTHSDKPYKC DRCQASFRYK GNLASHKTVH TGEKPYRCNI CGAQFNRPAN LKTHTRIHSG EKPY

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Hr4436B	[U-100% 13C; U-100% 15N]	0.8 mM
2	sodium azide	natural abundance	2 %
3	DTT	natural abundance	10 mM
4	ZnSO4	natural abundance	50 uM
5	DSS	natural abundance	50 uM
6	sodium chloride	natural abundance	100 mM
7	MES	natural abundance	20 mM
8	D20	natural abundance	5 %
9	H2O	natural abundance	95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	Hr4436B	[U-100% 15N]	0.65 mM
11	sodium azide	natural abundance	2 %
12	DTT	natural abundance	10 mM
13	ZnSO4	natural abundance	50 uM
14	DSS	natural abundance	50 uM
15	sodium chloride	natural abundance	100 mM
16	MES	natural abundance	20 mM
17	C12E5 PEG/Hexanol	natural abundance	4.2 %
18	D20	natural abundance	5 %
19	H2O	natural abundance	95 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	Hr4436B	[U-100% 13C; U-100% 15N]	0.8 mM
21	sodium azide	natural abundance	2 %
22	DTT	natural abundance	10 mM
23	ZnSO4	natural abundance	50 uM
24	DSS	natural abundance	50 uM
25	sodium chloride	natural abundance	100 mM
26	MES	natural abundance	20 mM
27	Positively Charged Stretch Polyacrylamide Gel	natural abundance	5 %
28	D20	natural abundance	5 %
29	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LCE, Strand ID: A Detail

Release date

2011-05-31

Citation

Solution Structure of Hr4436B
Lee, H., Montelione, G.T., Prestegard, J.H.
Not known

Related entities 1. Hr4436B, : 1 : 1 : 2416 entities Detail

Interaction partners 1. Hr4436B, : 39 interactors Detail

More details for 39 interactors identified by 11 citations