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BMRB: 17618

2LCN

1H and 15N assignments of WALP19-P10 peptide in SDS micelles

Authors

Vostrikov, V.V., Courtney, J.M., Hinton, J.F., Koeppe, R.E.

Assembly

WALP19-P10 complex

Entity

1. WALP19-P10 (polymer, Thiol state: not present), 19 monomers, 2093.511 Da Detail

GWWLALALAP ALALALWWA

2. ACE (non-polymer), 44.053 Da
3. ETA (non-polymer), 61.083 Da

Total weight

2198.647 Da

Max. entity weight

2093.511 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.6 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	95.6 % (129 of 135)	94.7 % (107 of 113)	100.0 % (22 of 22)
Backbone	100.0 % (56 of 56)	100.0 % (38 of 38)	100.0 % (18 of 18)
Sidechain	92.4 % (73 of 79)	92.0 % (69 of 75)	100.0 % (4 of 4)
Aromatic	89.3 % (25 of 28)	87.5 % (21 of 24)	100.0 % (4 of 4)
Methyl	100.0 % (19 of 19)	100.0 % (19 of 19)

1. WALP19-P10

GWWLALALAP ALALALWWA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Structural studies

#	Name	Isotope labeling	Concentration
1	WALP19-P10	natural abundance	7.1 mM
2	SDS	[U-100% 2H]	465 mM
3	TFE	[U-100% 2H]	3.4 %
4	H2O	natural abundance	88.3 %
5	D2O	natural abundance	8.3 %
6	potassium phosphate	natural abundance	32 uM

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Relaxation studies (selective 15N labeling)

#	Name	Isotope labeling	Concentration
7	WALP19-P10	[U-100% 15N]	3.2 mM
8	SDS	[U-100% 2H]	400 mM
9	TFE	[U-100% 2H]	3.4 %
10	H2O	natural abundance	88.3 %
11	D2O	natural abundance	8.3 %
12	potassium phosphate	natural abundance	32 uM

Sample #3

Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Relaxation studies (selective 15N labeling)

#	Name	Isotope labeling	Concentration
13	WALP19-P10	[U-100% 15N]	3.2 mM
14	SDS	[U-100% 2H]	400 mM
15	TFE	[U-100% 2H]	3.4 %
16	H2O	natural abundance	88.3 %
17	D2O	natural abundance	8.3 %
18	potassium phosphate	natural abundance	32 uM

Protein Blocks Logo

Calculated from 10 models in PDB: 2LCN, Strand ID: A Detail

Release date

2012-04-10

Citation

Comparison of Proline Substitutions at Positions 8 and 10 in WALP19
Vostrikov, V.V., Courtney, J.M., Hinton, J.F., Koeppe, R.E.
Not known

Related entities 1. WALP19-P10, : 1 : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Structural studies

#	Name	Isotope labeling	Concentration
1	WALP19-P10	natural abundance	7.1 mM
2	SDS	[U-100% 2H]	465 mM
3	TFE	[U-100% 2H]	3.4 %
4	H2O	natural abundance	88.3 %
5	D2O	natural abundance	8.3 %
6	potassium phosphate	natural abundance	32 uM

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Structural studies

#	Name	Isotope labeling	Concentration
1	WALP19-P10	natural abundance	7.1 mM
2	SDS	[U-100% 2H]	465 mM
3	TFE	[U-100% 2H]	3.4 %
4	H2O	natural abundance	88.3 %
5	D2O	natural abundance	8.3 %
6	potassium phosphate	natural abundance	32 uM

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 273 K, pH 6.0, Details Structural studies

#	Name	Isotope labeling	Concentration
1	WALP19-P10	natural abundance	7.1 mM
2	SDS	[U-100% 2H]	465 mM
3	TFE	[U-100% 2H]	3.4 %
4	H2O	natural abundance	88.3 %
5	D2O	natural abundance	8.3 %
6	potassium phosphate	natural abundance	32 uM

4 2D 1H-15N HSQC T1