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BMRB: 17636

2LV3

1H, 13C, and 15N NMR resonance assignments of reduced full length and shortened forms of the Grx domain of Mus musculus TGR

Authors

Shumilina, E., Solda, A., Gerashchenko, M., Gladyshev, V.N., Dikiy, A.

Assembly

Grx domain

Entity

1. Grx domain (polymer, Thiol state: all free), 124 monomers, 13907.58 Da Detail

MSSPPGRRAR LASPGTSRPS SEAREELRRR LRDLIEGNRV MIFSKSYCPH STRVKELFSS LGVVYNILEL DQVDDGASVQ EVLTEISNQK TVPNIFVNKV HVGGCDRTFQ AHQNGLLQKL LQDD

Formula weight

13907.58 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 90.1 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1290 of 1432)	90.6 % (681 of 752)	87.8 % (482 of 549)	96.9 % (127 of 131)
Backbone	91.1 % (667 of 732)	95.2 % (238 of 250)	86.5 % (315 of 364)	96.6 % (114 of 118)
Sidechain	90.0 % (734 of 816)	88.2 % (443 of 502)	92.4 % (278 of 301)	100.0 % (13 of 13)
Aromatic	88.2 % (60 of 68)	88.2 % (30 of 34)	88.2 % (30 of 34)
Methyl	98.5 % (134 of 136)	98.5 % (67 of 68)	98.5 % (67 of 68)

1. Grx domain of Mus musculus TGR

MSSPPGRRAR LASPGTSRPS SEAREELRRR LRDLIEGNRV MIFSKSYCPH STRVKELFSS LGVVYNILEL DQVDDGASVQ EVLTEISNQK TVPNIFVNKV HVGGCDRTFQ AHQNGLLQKL LQDD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	Grx domain of Mus musculus TGR	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	beta-mercaptoethanol	natural abundance	10 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LV3, Strand ID: A Detail

Release date

2011-09-12

Citation

¹H, ¹³C, and ¹⁵N NMR resonance assignments of reduced full length and shortened forms of the Grx domain of Mus musculus TGR
Shumilina, E., Solda, A., Gerashchenko, M., Gladyshev, V.N., Dikiy, A.
Biomol. NMR Assign. (2012), 6, 103-107, PubMed 21901408 , DOI 10.1007/s12104-011-9335-0 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17637 released on 2011-09-12
Title 1H, 13C, and 15N NMR resonance assignments of reduced full length and shortened forms of the Grx domain of Mus musculus TGR

Related entities 1. Grx domain, : 1 : 1 : 48 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC