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BMRB: 17638

2LCY

NMR Structure of the Complete Internal Fusion Loop from Ebolavirus GP2 at pH 5.5

Authors

Gregory, S.M., Harada, E., Liang, B., Tamm, L.K.

Assembly

Ebolavirus Fusion Loop pH 5.5

Entity

1. Ebolavirus Fusion Loop pH 5.5 (polymer, Thiol state: all disulfide bound), 54 monomers, 5943.657 Da Detail

AQPKCNPNLH YWTTQDEGAA IGLAWIPYFG PAAEGIYIEG LMHNQDGLIC GLRQ

Formula weight

5943.657 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 77.1 %, Completeness (bb): 81.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.1 % (477 of 619)	89.7 % (287 of 320)	58.7 % (141 of 240)	83.1 % (49 of 59)
Backbone	81.6 % (258 of 316)	100.0 % (111 of 111)	63.2 % (98 of 155)	98.0 % (49 of 50)
Sidechain	74.9 % (262 of 350)	84.2 % (176 of 209)	65.2 % (86 of 132)	0.0 % (0 of 9)
Aromatic	78.8 % (52 of 66)	93.9 % (31 of 33)	67.7 % (21 of 31)	0.0 % (0 of 2)
Methyl	62.5 % (35 of 56)	89.3 % (25 of 28)	35.7 % (10 of 28)

1. Ebolavirus Fusion Loop pH 5.5

AQPKCNPNLH YWTTQDEGAA IGLAWIPYFG PAAEGIYIEG LMHNQDGLIC GLRQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Ebolavirus Fusion Loop pH 5.5	[U-13C; U-15N]	0.5-1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium phosphate	natural abundance	30 mM
5	sodium chloride	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
6	Ebolavirus Fusion Loop pH 5.5	[U-15N]	0.5-1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	sodium phosphate	natural abundance	30 mM
10	sodium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LCY, Strand ID: A Detail

Release date

2011-08-02

Citation

Structure and function of the complete internal fusion loop from Ebolavirus glycoprotein 2
Gregory, S.M., Harada, E., Liang, B., Delos, S.E., White, J.M., Tamm, L.K.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 11211-11216, PubMed 21690393 , DOI 10.1073/pnas.1104760108 , Abstract

Related entities 1. Ebolavirus Fusion Loop pH 5.5, : 1 : 11 : 9 : 17 entities Detail

Interaction partners 1. Ebolavirus Fusion Loop pH 5.5, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC