1H, 13C & 13N chemical shift assignments for Toxoplasma gondii Microneme protein 4 (MIC4) residues 410-491 (apple domain 5)
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS10:SG | 1:CYS79:SG |
2 | disulfide | sing | 1:CYS35:SG | 1:CYS57:SG |
3 | disulfide | sing | 1:CYS39:SG | 1:CYS45:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.5 % (782 of 915) | 82.7 % (396 of 479) | 89.5 % (315 of 352) | 84.5 % (71 of 84) |
Backbone | 89.5 % (435 of 486) | 86.9 % (146 of 168) | 91.6 % (219 of 239) | 88.6 % (70 of 79) |
Sidechain | 82.5 % (416 of 504) | 80.4 % (250 of 311) | 87.8 % (165 of 188) | 20.0 % (1 of 5) |
Aromatic | 72.2 % (52 of 72) | 72.2 % (26 of 36) | 72.2 % (26 of 36) | |
Methyl | 87.5 % (49 of 56) | 85.7 % (24 of 28) | 89.3 % (25 of 28) |
1. TgMIC4-apple5
SPDFHDEVEC VHTGNIGSKA QTIGEVKRAS SLSECRARCQ AEKECSHYTY NVKSGLCYPK RGKPQFYKYL GDMTGSRTCD TSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TgMIC4-apple5 | [U-98% 13C; U-98% 15N] | 100.0 ~ 200.0 mM | |
2 | potassium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H20 | natural abundance | 90 % | |
5 | D20 | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | TgMIC4-apple5 | [U-98% 13C; U-98% 15N] | 100-200 mM | |
7 | potassium phosphate | natural abundance | 50 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | H20 | natural abundance | 90 % | |
10 | D20 | natural abundance | 10 % |