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Found 1 Document (1.136 seconds)

BMRB: 17680

2LDR

Solution structure of Helix-RING domain in the Tyr363 phosphorylated form.

Authors

Kumeta, H., Kobashigawa, Y., Inagaki, F.

Assembly

CBLB-pY-H-RING

Entity

1. CBLB-pY-H-RING (polymer, Thiol state: free and other bound), 82 monomers, 9255.390 Da Detail

GPHMGSDHIK VTQEQYELXC EMGSTFQLCK ICAENDKDVK IEPCGHLMCT SCLTAWQESD GQGCPFCRCE IKGTEPIIVD PF

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9386.208 Da

Max. entity weight

9255.39 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 93.4 %, Completeness (bb): 93.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.4 % (852 of 912)	91.4 % (435 of 476)	94.9 % (335 of 353)	98.8 % (82 of 83)
Backbone	93.3 % (444 of 476)	86.6 % (142 of 164)	96.2 % (227 of 236)	98.7 % (75 of 76)
Sidechain	94.3 % (481 of 510)	93.9 % (293 of 312)	94.8 % (181 of 191)	100.0 % (7 of 7)
Aromatic	87.1 % (54 of 62)	100.0 % (31 of 31)	73.3 % (22 of 30)	100.0 % (1 of 1)
Methyl	93.9 % (62 of 66)	87.9 % (29 of 33)	100.0 % (33 of 33)

1. CBLB-pY-H-RING

GPHMGSDHIK VTQEQYELXC EMGSTFQLCK ICAENDKDVK IEPCGHLMCT SCLTAWQESD GQGCPFCRCE IKGTEPIIVD PF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	CBLB-pY-H-RING	[U-99% 13C; U-99% 15N]	0.4 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LDR, Strand ID: A Detail

Release date

2012-02-21

Citation

Autoinhibition and phosphorylation-induced activation mechanisms of human cancer and autoimmune disease-related E3 protein Cbl-b
Kobashigawa, Y., Tomitaka, A., Kumeta, H., Noda, N.N., Yamaguchi, M., Inagaki, F.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 20579-20584, PubMed 22158902 , DOI 10.1073/pnas.1110712108 , Abstract

Related entities 1. CBLB-pY-H-RING, : 1 : 50 entities Detail

Interaction partners 1. CBLB-pY-H-RING, : 34 interactors Detail

More details for 34 interactors identified by 20 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC