BMRB: 17685

Solution structure of the C-terminal Pdr1 activating domain of Zuo1

Authors

Volkman, B.F., Waltner, J.K., Peterson, F.C.

Assembly

Zuo1

Entity

1. Zuo1 (polymer, Thiol state: not present), 108 monomers, 11812.01 Da Detail

MRGSHHHHHH HHGSENLYFQ GSKAAKKKNK RAIRNSAKEA DYFGDADKAT TIDEQVGLIV DSLNDEELVS TADKIKANAA GAKEVLKESA KTIVDSGKLP SSLLSYFV

Formula weight

11812.01 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.5 %, Completeness: 79.6 %, Completeness (bb): 81.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.6 % (970 of 1218)	80.0 % (505 of 631)	78.9 % (373 of 473)	80.7 % (92 of 114)
Backbone	81.1 % (524 of 646)	80.2 % (178 of 222)	81.7 % (259 of 317)	81.3 % (87 of 107)
Sidechain	78.6 % (529 of 673)	80.0 % (327 of 409)	76.7 % (197 of 257)	71.4 % (5 of 7)
Aromatic	32.6 % (28 of 86)	41.9 % (18 of 43)	23.3 % (10 of 43)
Methyl	96.4 % (106 of 110)	96.4 % (53 of 55)	96.4 % (53 of 55)

1. Zuo1

MRGSHHHHHH HHGSENLYFQ GSKAAKKKNK RAIRNSAKEA DYFGDADKAT TIDEQVGLIV DSLNDEELVS TADKIKANAA GAKEVLKESA KTIVDSGKLP SSLLSYFV

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 1.2 mM Zuo1 (348-433) U-15N/13C, 20 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT

#	Name	Isotope labeling	Type	Concentration
1	Zuo1	[U-100% 13C; U-100% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	DTT	natural abundance		1 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.24 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LWX, Strand ID: A Detail

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Release date

2012-09-30

Citation

Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription
Ducett, J.K., Peterson, F.C., Hoover, L.A., Prunuske, A.J., Volkman, B.F., Craig, E.A.
J. Mol. Biol. (2013), 425, 19-31, PubMed 23036859 , DOI 10.1016/j.jmb.2012.09.020 , Abstract

Related entities 1. Zuo1, : 3 entities Detail

Experiments performed 3 experiments Detail