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BMRB: 17686

2LDY

Solution structure of the RMM-CTD domains of human LINE-1 ORF1p

Authors

Coles, M., Truffault, V.

Assembly

L1ORF1p

Entity

1. L1ORF1p (polymer, Thiol state: not present), 182 monomers, 21476.51 Da Detail

MGNLRLIGVP ESDVENGTKL ENTLQDIIQE NFPNLARQAN VQIQEIQRTP QRYSSRRATP RHIIVRFTKV EMKEKMLRAA REKGRVTLKG KPIRLTVDLS AETLQARREW GPIFNILKEK NFQPRISYPA KLSFISEGEI KYFIDKQMLR DFVTTRPALK ELLKEALNME RNNRYQHHHH HH

Formula weight

21476.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 86.0 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.0 % (1944 of 2261)	87.7 % (1054 of 1202)	84.2 % (727 of 863)	83.2 % (163 of 196)
Backbone	87.9 % (944 of 1074)	89.5 % (324 of 362)	87.0 % (469 of 539)	87.3 % (151 of 173)
Sidechain	85.5 % (1165 of 1362)	86.9 % (730 of 840)	84.8 % (423 of 499)	52.2 % (12 of 23)
Aromatic	51.4 % (73 of 142)	81.7 % (58 of 71)	20.0 % (14 of 70)	100.0 % (1 of 1)
Methyl	96.9 % (190 of 196)	95.9 % (94 of 98)	98.0 % (96 of 98)

1. L1ORF1p

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
1	L1ORF1p-RMM	[U-100% 15N]	0.6 mM
2	Tris	natural abundance	5 mM
3	sodium chloride	natural abundance	300 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
6	L1ORF1p-RMM	[U-100% 13C; U-100% 15N]	0.8 mM
7	Tris	natural abundance	5 mM
8	sodium chloride	natural abundance	300 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
11	L1ORF1p-CTD	[U-100% 15N]	0.8 mM
12	Tris	natural abundance	5 mM
13	sodium chloride	natural abundance	300 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
16	L1ORF1p-CTD	[U-100% 13C; U-100% 15N]	0.8 mM
17	Tris	natural abundance	5 mM
18	sodium chloride	natural abundance	300 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
21	L1ORF1p-RMM-CTD	[U-100% 15N]	0.8 mM
22	Tris	natural abundance	5 mM
23	sodium chloride	natural abundance	300 mM
24	H2O	natural abundance	90 %
25	D2O	natural abundance	10 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 8.0

#	Name	Isotope labeling	Concentration
26	L1ORF1p-RMM-CTD	[U-100% 13C; U-100% 15N]	0.8 mM
27	Tris	natural abundance	5 mM
28	sodium chloride	natural abundance	300 mM
29	H2O	natural abundance	90 %
30	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	2.6 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 2LDY, Strand ID: A Detail

Release date

2012-02-21

Citation

Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition
Khazina, E., Truffault, V., Buttner, R., Schmidt, S., Coles, M., Weichenrieder, O.
Nat. Struct. Mol. Biol. (2011), 18, 1006-1014, PubMed 21822284 , DOI 10.1038/nsmb.2097 , Abstract

Related entities 1. L1ORF1p, : 1 : 2 : 42 entities Detail

Interaction partners 1. L1ORF1p, : 12 interactors Detail

More details for 12 interactors identified by 2 citations

Experiments performed 32 experiments Detail

1 3D 1H-15N NOESY