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BMRB: 17693

2YKA

RRM domain of mRNA export adaptor REF2-I bound to HVS ORF57 peptide

Authors

Tunnicliffe, R.BRYAN., Hautbergue, G.M., Kalra, P., Wilson, S.A., Golovanov, A.P., Golova, A.

Assembly

RRM domain of mRNA export adaptor REF2-I bound to HVS ORF57 peptide

Entity

1. RNA AND EXPORT FACTOR-BINDING PROTEIN 2 (polymer, Thiol state: all free), 124 monomers, 13574.96 Da Detail

MASMTGGQQM GRDPDKWQHD LFDSGCGGGE GVETGAKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVDYD RSGRSLGTAD VHFERRADAL KAMKQYKGVP LDGRPMDIQL VASQIDLEHH HHHH

2. 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN (polymer, Thiol state: all free), 23 monomers, 2502.767 Da Detail

GPLGSSCKTS WADRVREAAA QRR

Total weight

16077.727 Da

Max. entity weight

13574.96 Da

Source organism

Saimiriine gammaherpesvirus 2 , Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.3 %, Completeness: 83.7 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (1358 of 1623)	86.2 % (727 of 843)	79.9 % (500 of 626)	85.1 % (131 of 154)
Backbone	82.4 % (720 of 874)	84.4 % (259 of 307)	80.4 % (341 of 424)	83.9 % (120 of 143)
Sidechain	84.6 % (744 of 879)	87.3 % (468 of 536)	79.8 % (265 of 332)	100.0 % (11 of 11)
Aromatic	69.7 % (85 of 122)	77.0 % (47 of 61)	61.0 % (36 of 59)	100.0 % (2 of 2)
Methyl	93.9 % (124 of 132)	97.0 % (64 of 66)	90.9 % (60 of 66)

1. RNA AND EXPORT FACTOR-BINDING PROTEIN 2

MASMTGGQQM GRDPDKWQHD LFDSGCGGGE GVETGAKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVDYD RSGRSLGTAD VHFERRADAL KAMKQYKGVP LDGRPMDIQL VASQIDLEHH HHHH

2. 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN

GPLGSSCKTS WADRVREAAA QRR

Show sample condition

Sample #1

Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

Sample #2

Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

Chem. Shift Complete2

Sequence coverage: 31.3 %, Completeness: 52.5 %, Completeness (bb): 54.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.5 % (1705 of 3246)	53.4 % (900 of 1686)	50.7 % (635 of 1252)	55.2 % (170 of 308)
Backbone	54.1 % (946 of 1748)	56.0 % (344 of 614)	52.2 % (443 of 848)	55.6 % (159 of 286)
Sidechain	50.9 % (895 of 1758)	51.9 % (556 of 1072)	49.4 % (328 of 664)	50.0 % (11 of 22)
Aromatic	38.9 % (95 of 244)	42.6 % (52 of 122)	34.7 % (41 of 118)	50.0 % (2 of 4)
Methyl	61.0 % (161 of 264)	62.9 % (83 of 132)	59.1 % (78 of 132)

1. RNA AND EXPORT FACTOR-BINDING PROTEIN 2

MASMTGGQQM GRDPDKWQHD LFDSGCGGGE GVETGAKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVDYD RSGRSLGTAD VHFERRADAL KAMKQYKGVP LDGRPMDIQL VASQIDLEHH HHHH

2. 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN

GPLGSSCKTS WADRVREAAA QRR

Show sample condition

Sample #1

Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

Sample #2

Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2YKA, Strand ID: A, B Detail

Release date

2012-06-10

Citation

Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57
Tunnicliffe, R.BRYAN., Hautbergue, G.M., Kalra, P., Jackson, B.R., Whitehouse, A., Wilson, S.A., Golovanov, A.P.
PLoS Pathog. (2011), 7, e1001244-e1001244, PubMed 21253573 , DOI 10.1371/journal.ppat.1001244 , Abstract

Related entities 1. RNA AND EXPORT FACTOR-BINDING PROTEIN 2, : 31 entities Detail

Related entities 2. 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN, : 1 : 1 entities Detail

Experiments performed 21 experiments Detail

1 HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

2 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

3 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

4 HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

5 HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

6 HN(CA)CO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

7 TOCSY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

8 HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

9 HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

10 CCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

11 NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

12 Filtered 13C-NOESY

Instrument

Varian Inova - 800 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

13 HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

14 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

15 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

16 HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

17 HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

18 TOCSY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

19 HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

20 NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details ORF57 1 mM, REF 3 mM

#	Name	Isotope labeling	Concentration
4	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	3 mM
5	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	1 mM
6	NaCl	natural abundance	50 mM

21 NOESY-HSQC HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 303.000 K, pH 6.200, Details REF 1 mM, ORF57 3 mM

#	Name	Isotope labeling	Concentration
1	RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2	[U-99% 13C; U-99% 15N]	1 mM
2	52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN	[U-99% 13C; U-99% 15N]	3 mM
3	NaCl	natural abundance	50 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_17693_2yka.nef
Input source #2: Coordindates	2yka.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

40-------50--------60--------70--------80--------90-------100-------110-------120-------130-------14
MASMTGGQQMGRDPDKWQHDLFDSGCGGGEGVETGAKLLVSNLDFGVSDADIQELFAEFGTLKKAAVDYDRSGRSLGTADVHFERRADALKAMKQYKGVP
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MASMTGGQQMGRDPDKWQHDLFDSGCGGGEGVETGAKLLVSNLDFGVSDADIQELFAEFGTLKKAAVDYDRSGRSLGTADVHFERRADALKAMKQYKGVP
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

0-------150-------160---
LDGRPMDIQLVASQIDLEHHHHHH
||||||||||||||||||||||||
LDGRPMDIQLVASQIDLEHHHHHH
-------110-------120----

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--100-----110-------120
GPLGSSCKTSWADRVREAAAQRR
|||||||||||||||||||||||
GPLGSSCKTSWADRVREAAAQRR
--------10--------20---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	124	0	0	100.0
B	B	23	0	0	100.0

Content subtype: combined_17693_2yka.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1345		96.0 (chain: A, length: 124), 100.0 (chain: B, length: 23)
2	Assigned chemical shifts	assigned_chem_shift_list_1_2	Warning	131		16.9 (chain: A, length: 124)
1	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	62 (1)	noe	8.9 (chain: A, length: 124), 39.1 (chain: B, length: 23)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	OK	164 (164)	.	72.6 (chain: A, length: 124), 60.9 (chain: B, length: 23)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 124, Sequence coverage: 96.0%
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 485
  - ¹H chemical shifts: 725
  - ¹⁵N chemical shifts: 135
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B
Saveframe: assigned_chem_shift_list_1_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 124, Sequence coverage: 16.9%
- Total number of assignments
  - ¹³C chemical shifts: 50
  - ¹H chemical shifts: 63
  - ¹⁵N chemical shifts: 18
- Completeness of assignments
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
124	ARG	HH11	6.931
143	ARG	HH11	6.535
143	ARG	HH12	6.652

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	533	448	84.1
¹H chemical shifts	712	649	91.2
¹⁵N chemical shifts	136	126	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	248	217	87.5
¹H chemical shifts	260	242	93.1
¹⁵N chemical shifts	121	113	93.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	285	231	81.1
¹H chemical shifts	452	407	90.0
¹⁵N chemical shifts	15	13	86.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	62	57	91.9
¹H chemical shifts	62	59	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	54	30	55.6
¹H chemical shifts	55	39	70.9
¹⁵N chemical shifts	1	1	100.0

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
111	ARG	HH11	6.88
111	ARG	HH21	6.805
113	ARG	HH11	6.492
113	ARG	HH12	6.575
113	ARG	HH21	6.806
113	ARG	HH22	6.883

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	93	37	39.8
¹H chemical shifts	131	67	51.1
¹⁵N chemical shifts	28	9	32.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	46	11	23.9
¹H chemical shifts	47	12	25.5
¹⁵N chemical shifts	22	3	13.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	47	26	55.3
¹H chemical shifts	84	55	65.5
¹⁵N chemical shifts	6	6	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	9	6	66.7
¹H chemical shifts	9	8	88.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	5	4	80.0
¹H chemical shifts	6	6	100.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 124, Sequence coverage: 8.9%
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 39.1%
- Total number of restraints: 62
- Weight of restraints: 1.0 (62)
- Potential type of restraints: upper-bound-parabolic (62)
- Range of distance target values: 4.0, 5.5 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 124, Sequence coverage: 72.6%
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 60.9%
- Total number of restraints: 164
- Total number of restraints per polymer type: 164
- Weight of restraints: 1.0 (164)
- Potential type of restraints: square-well-parabolic (164)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords mRNA export, RNA BINDING PROTE, RNA binding protein, RNA BINDING PROTEIN-TRANSCRIPTION COMPLEX

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Found 1 Document (0.953 seconds)

BMRB: 17693

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Related entities 1. RNA AND EXPORT FACTOR-BINDING PROTEIN 2, : 31 entities Detail

Related entities 2. 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN, : 1 : 1 entities Detail

Experiments performed 21 experiments Detail

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NMR combined restraints 5 contents Detail