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BMRB: 17694

2LS6

1H, 15N and 13C backbone and side-chain assignments of a family 32 carbohydrate-binding module (CBM32) from the Clostridium perfringens NagH

Authors

Grondin, J.M., Chitayat, S., Ficko-Blean, E., Boraston, A.B., Smith, S.P.

Assembly

GH84A CBM32-1

Entity

1. GH84A CBM32-1 (polymer, Thiol state: not present), 164 monomers, 18328.14 Da Detail

MGSSHHHHHH SSGLVPRGSH MASDPNRIIA TYISNRQDAP TGNPDNIFDN NASTELVYKN PNRIDVGTYV GVKYSNPITL NNVEFLMGAN SNPNDTMQKA KIQYTVDGRE WIDLEEGVEY TMPGAIKVEN LDLKVRGVRL IATEARENTW LGVRDINVNK KEDS

Formula weight

18328.14 Da

Source organism

Clostridium perfringens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 86.3 %, Completeness (bb): 88.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.3 % (1606 of 1862)	85.7 % (830 of 968)	85.9 % (614 of 715)	90.5 % (162 of 179)
Backbone	88.9 % (861 of 968)	89.2 % (296 of 332)	88.5 % (425 of 480)	89.7 % (140 of 156)
Sidechain	84.3 % (882 of 1046)	84.0 % (534 of 636)	84.2 % (326 of 387)	95.7 % (22 of 23)
Aromatic	43.3 % (52 of 120)	43.3 % (26 of 60)	41.4 % (24 of 58)	100.0 % (2 of 2)
Methyl	97.6 % (166 of 170)	97.6 % (83 of 85)	97.6 % (83 of 85)

1. GH84A CBM32-1

MGSSHHHHHH SSGLVPRGSH MASDPNRIIA TYISNRQDAP TGNPDNIFDN NASTELVYKN PNRIDVGTYV GVKYSNPITL NNVEFLMGAN SNPNDTMQKA KIQYTVDGRE WIDLEEGVEY TMPGAIKVEN LDLKVRGVRL IATEARENTW LGVRDINVNK KEDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	GH84A_CBM32-1	[U-100% 13C; U-100% 15N]	0.8 mM
2	H20	natural abundance	90 %
3	D20	natural abundance	10 %
4	TRIS	natural abundance	25 mM
5	sodium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 2LS6, Strand ID: A Detail

Release date

2011-09-13

Citation

1H, 15N and 13C backbone and side-chain resonance assignments of a family 32 carbohydrate-binding module from the Clostridium perfringens NagH
Grondin, J.M., Chitayat, S., Ficko-Blean, E., Boraston, A.B., Smith, S.P.
Biomol. NMR Assign. (2012), 6, 139-142, PubMed 21912839 , DOI 10.1007/s12104-011-9342-1 , Abstract

Related entities 1. GH84A CBM32-1, : 1 : 1 : 2 entities Detail

Interaction partners 1. GH84A CBM32-1, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC