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BMRB: 17703

2LTR

Backbone and stereo-specific Ile(d1), Leu, Val methyl sidechain chemical shift assignments of RDE-4 (1-243)

Authors

Chiliveri, S., Kumar, S., Marelli, U., Deshmukh, M.V.

Assembly

RDE-4DC

Entity

1. RDE-4DC (polymer, Thiol state: all free), 251 monomers, 28505.94 Da Detail

MDLTKLTFES VFGGSDVPMK PSRSEDNKTP RNRTDLEMFL KKTPLMVLEE AAKAVYQKTP TWGTVELPEG FEMTLILNEI TVKGQATSKK AARQKAAVEY LRKVVEKGKH EIFFIPGTTK EEALSNIDQI SDKAEELKRS TSDAVQDNDN DDSIPTSAEF PPGISPTENW VGKLQEKSQK SKLQAPIYED SKNERTERFL VICTMCNQKT RGIRSKKKDA KNLAAWLMWK ALEDGIESLE SYDLEHHHHH H

Formula weight

28505.94 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 53.3 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.3 % (1584 of 2971)	33.2 % (518 of 1561)	72.4 % (831 of 1148)	89.7 % (235 of 262)
Backbone	81.8 % (1213 of 1482)	54.9 % (275 of 501)	95.6 % (709 of 742)	95.8 % (229 of 239)
Sidechain	34.6 % (599 of 1729)	22.9 % (243 of 1060)	54.2 % (350 of 646)	26.1 % (6 of 23)
Aromatic	11.7 % (22 of 188)	17.0 % (16 of 94)	2.2 % (2 of 90)	100.0 % (4 of 4)
Methyl	68.1 % (169 of 248)	62.9 % (78 of 124)	73.4 % (91 of 124)

1. RDE-4DC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	RDE-4DC	[U-100% 15N]	200 uM
2	RDE-4DC	[U-15N]-Lys	200 uM
3	RDE-4DC	[U-15N]-(-R)Arg	200 uM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %
6	Potassium phosphate buffer	natural abundance	50 mM
7	Na2SO4	natural abundance	100 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	RDE-4DC	[U-100% 13C; U-100% 15N; U-80% 2H]	500 uM
9	H20	natural abundance	90 %
10	D20	natural abundance	10 %
11	Potassium phosphate buffer	natural abundance	50 mM
12	Na2SO4	natural abundance	100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	RDE-4DC	[U-13C Ile, Leu, Val (rest 12C); U-15N; U-2H]	500 uM
14	H20	natural abundance	90 %
15	D20	natural abundance	10 %
16	Potassium phosphate buffer	natural abundance	50 mM
17	Na2SO4	natural abundance	100 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
18	RDE-4DC	[U-13C,1H Me only Ile, Leu, Val (rest 12C,2H); 15N,1H, Phe; U-15N; U-2H]	500 uM
19	RDE-4DC	[U-13C,1H Me only Ile, Leu, Val (rest 12C,2H); 15N,1H, Tyr; U-15N; U-2H]	500 uM
20	H20	natural abundance	90 %
21	D20	natural abundance	10 %
22	Potassium phosphate buffer	natural abundance	50 mM
23	Na2SO4	natural abundance	100 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
24	RDE-4DC	[U-10% 13C]	200 uM
25	H20	natural abundance	90 %
26	D20	natural abundance	10 %
27	Potassium phosphate buffer	natural abundance	50 mM
28	Na2SO4	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.75 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.75 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LTR, Strand ID: A Detail

Release date

2011-10-25

Citation

Backbone and sidechain methyl Ile (δ1), Leu and Val chemical shift assignments of RDE-4 (1-243), an RNA interference initiation protein in C. elegans
Chiliveri, S., Kumar, S., Marelli, U., Deshmukh, M.V.
Biomol. NMR Assign. (2012), 6, 143-146, PubMed 22002349 , DOI 10.1007/s12104-011-9343-0 , Abstract

Related entities 1. RDE-4DC, : 2 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC