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RAGEC2-S100A13 tetrameric complex
Authors
Rani, S.G., Mohan, S.K., Yu, C.
Assembly
RAGEC2-S100A13 tetrameric complex
Entity
1. RAGEC2-S100A13 tetrameric complex, entity 1 (polymer), 93 monomers, 9640.749 × 2 Da Detail

LEEVQLVVEP EGGAVAPGGT VTLTCEVPAQ PSPQIHWMKD GVPLPLPPSP VLILPEIGPQ DQGTYSCVAT HSSHGPQESR AVSISIIEPG EEG


2. RAGEC2-S100A13 tetrameric complex, entity 2 (polymer), 97 monomers, 11339.84 × 2 Da Detail

AAEPLTELEE SIETVVTTFF TFARQEGRKD SLSVNEFKEL VTQQLPHLLK DVGSLDEKMK SLDVNQDSEL KFNEYWRLIG ELAKEIRKKK DLKIRKK


Total weight
41961.18 Da
Max. entity weight
11339.84 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 51.1 %, Completeness: 40.6 %, Completeness (bb): 46.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.6 % (897 of 2210)39.9 % (462 of 1157)41.3 % (357 of 864)41.3 % (78 of 189)
Backbone46.2 % (512 of 1108)47.7 % (180 of 377)45.6 % (254 of 557)44.8 % (78 of 174)
Sidechain36.4 % (465 of 1279)36.2 % (282 of 780)37.8 % (183 of 484) 0.0 % (0 of 15)
Aromatic 0.0 % (0 of 106) 0.0 % (0 of 53) 0.0 % (0 of 51) 0.0 % (0 of 2)
Methyl48.2 % (108 of 224)49.1 % (55 of 112)47.3 % (53 of 112)

1. entity 1

LEEVQLVVEP EGGAVAPGGT VTLTCEVPAQ PSPQIHWMKD GVPLPLPPSP VLILPEIGPQ DQGTYSCVAT HSSHGPQESR AVSISIIEPG EEG

2. entity 2

AAEPLTELEE SIETVVTTFF TFARQEGRKD SLSVNEFKEL VTQQLPHLLK DVGSLDEKMK SLDVNQDSEL KFNEYWRLIG ELAKEIRKKK DLKIRKK

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C&15N labeled RAGEC2 and Unlabeled S100A13 (1:1)


#NameIsotope labelingTypeConcentration
1entity_1[U-100% 13C; U-100% 15N]1.1 mM
2entity_2natural abundance1.1 mM
3sodium phosphatenatural abundance25 mM
4sodium chloridenatural abundance100 mM
5DTTnatural abundance1 mM
6sodium azidenatural abundance0.02 mM
7H2Onatural abundance90 %
8D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C &15N labeled S100A13 with Unlabeled RAGEC2 domain (1:1)


#NameIsotope labelingTypeConcentration
9entity_1natural abundance1.2 mM
10entity_2[U-100% 13C; U-100% 15N]1.2 mM
11sodium phosphatenatural abundance25 mM
12sodium chloridenatural abundance100 mM
13DTTnatural abundance1 mM
14sodium azidenatural abundance0.02 mM
15H2Onatural abundance90 %
16D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: 2.7 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 2.7 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.04 ppm, Outliers: 4 Detail
LACS Plot; CO
Referencing offset: 3.47 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 2LE9, Strand ID: A, B, C, D Detail


Release date
2015-06-25
Citation
Interaction of S100A13 with C2 domain of receptor for advanced glycation end products (RAGE)
Rani, S.G., Mohan, S.K., Yu, C.
Biochim. Biophys. Acta (2014), 1844, 1718-1728, PubMed 24982031 , DOI 10.1016/j.bbapap.2014.06.017 ,
Related entities 1. RAGEC2-S100A13 tetrameric complex, entity 1, : 1 : 2 : 4 : 296 entities Detail
Related entities 2. RAGEC2-S100A13 tetrameric complex, entity 2, : 1 : 45 entities Detail
Interaction partners 1. RAGEC2-S100A13 tetrameric complex, entity 1, : 15 interactors Detail
Experiments performed 25 experiments Detail
nullKeywords Receptor for Advanced Glycation End products, S100A13, tetrameric complex