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Fpr4p PPIase domain
Authors
Monneau, Y.R., Mackereth, C.D.
Assembly
Fpr4p(280-392)
Entity
1. Fpr4p(280-392) (polymer, Thiol state: not present), 117 monomers, 12479.66 Da Detail

GAMAKPKTKL LEGGIIIEDR VTGKGPHAKK GTRVGMRYVG KLKNGKVFDK NTKGKPFVFK LGQGEVIKGW DIGVAGMAVG GERRIVIPAP YAYGKQALPG IPANSELTFD VKLVSMK


Formula weight
12479.66 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Refine. method
ARIA1.2
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 98.8 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All98.8 % (1353 of 1369)98.5 % (709 of 720)99.1 % (528 of 533)100.0 % (116 of 116)
Backbone98.5 % (678 of 688)98.0 % (241 of 246)98.5 % (327 of 332)100.0 % (110 of 110)
Sidechain99.2 % (773 of 779)98.7 % (468 of 474)100.0 % (299 of 299)100.0 % (6 of 6)
Aromatic100.0 % (80 of 80)100.0 % (40 of 40)100.0 % (39 of 39)100.0 % (1 of 1)
Methyl100.0 % (132 of 132)100.0 % (66 of 66)100.0 % (66 of 66)

1. Fpr4p

GAMAKPKTKL LEGGIIIEDR VTGKGPHAKK GTRVGMRYVG KLKNGKVFDK NTKGKPFVFK LGQGEVIKGW DIGVAGMAVG GERRIVIPAP YAYGKQALPG IPANSELTFD VKLVSMK

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 292 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Fpr4p[U-99% 15N]0.2 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance150 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 292 K, pH 6.5


#NameIsotope labelingTypeConcentration
6Fpr4p[U-99% 13C; U-99% 15N]0.2 mM
7sodium phosphatenatural abundance20 mM
8sodium chloridenatural abundance150 mM
9H2Onatural abundance90 %
10D2Onatural abundance10 %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 292 K, pH 6.5


#NameIsotope labelingTypeConcentration
11Fpr4p[U-99% 13C; U-99% 15N]0.2 mM
12sodium phosphatenatural abundance20 mM
13sodium chloridenatural abundance150 mM
14H2Onatural abundance90 %
15D2Onatural abundance10 %
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 292 K, pH 6.5


#NameIsotope labelingTypeConcentration
16Fpr4p[U-10% 13C; U-99% 15N]0.2 mM
17sodium phosphatenatural abundance20 mM
18sodium chloridenatural abundance150 mM
19H2Onatural abundance90 %
20D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.4 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.4 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.08 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.49 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 12 models in PDB: 4BF8, Strand ID: A Detail


Release date
2011-09-12
Citation
Chemical shift assignments of the catalytic domain from the yeast proline isomerase Fpr4p
Monneau, Y.R., Nelson, C.J., Mackereth, C.D.
Biomol. NMR Assign. (2012), 6, 123-126, PubMed 21898050 , DOI 10.1007/s12104-011-9338-x ,
Related entities 1. Fpr4p(280-392), : 1 : 2 : 153 entities Detail
Interaction partners 1. Fpr4p(280-392), : 56 interactors Detail
Experiments performed 17 experiments Detail
nullKeywords epigenetics, histone, proline isomerization, yeast