BMRBj - BMREntryMaster

Found 1 Document (0.895 seconds)

BMRB: 17732

2LEX

Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA

Authors

Kigawa, T., Watanabe, S., Inoue, M., Yokoyama, S.

Assembly

Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA

Entity

1. WRKY domain (polymer, Thiol state: not present), 78 monomers, 8572.404 Da Detail

GSSGSSGVQT TSEVDLLDDG YRWRKYGQKV VKGNPYPRSY YKCTTPGCGV RKHVERAATD PKAVVTTYEG KHNHDLPA

2. ZN (non-polymer, Thiol state: not present), 65.409 Da
3. W-box DNA chain 1 (polymer, Thiol state: not present), 16 monomers, 4898.102 Da Detail

CGCCTTTGAC CAGCGC

4. W-box DNA chain 2 (polymer, Thiol state: not present), 16 monomers, 5027.188 Da Detail

GCGCTGGTCA AAGGCG

Total weight

18563.104 Da

Max. entity weight

8572.404 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 64.7 %, Completeness (bb): 59.5 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	64.7 % (765 of 1183)	78.9 % (605 of 767)	25.7 % (87 of 338)	93.6 % (73 of 78)
Backbone	59.5 % (406 of 682)	66.7 % (256 of 384)	36.4 % (82 of 225)	93.2 % (68 of 73)
Sidechain	63.9 % (364 of 570)	91.1 % (349 of 383)	5.5 % (10 of 182)	100.0 % (5 of 5)
Aromatic	69.9 % (95 of 136)	94.0 % (94 of 100)	0.0 % (0 of 35)	100.0 % (1 of 1)
Methyl	49.3 % (35 of 71)	86.8 % (33 of 38)	6.1 % (2 of 33)

1. WRKY domain

GSSGSSGVQT TSEVDLLDDG YRWRKYGQKV VKGNPYPRSY YKCTTPGCGV RKHVERAATD PKAVVTTYEG KHNHDLPA

3. W-box DNA chain 1

CGCCTTTGAC CAGCGC

4. W-box DNA chain 2

GCGCTGGTCA AAGGCG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	WRKY_domain	natural abundance	0.4 ~ 1.0 mM
2	W-box DNA chain 1	natural abundance	0.4 ~ 1.0 mM
3	W-box DNA chain 2	natural abundance	0.4 ~ 1.0 mM
4	potassium phosphate	natural abundance	20 mM
5	potassium chloride	natural abundance	200 mM
6	zinc chloride	natural abundance	20 uM
7	DTT	[U-2H]	1 mM
8	DSS	natural abundance	50 uM
9	H2O	natural abundance	95 %
10	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	WRKY_domain	natural abundance	0.4 ~ 1.0 mM
12	W-box DNA chain 1	natural abundance	0.4 ~ 1.0 mM
13	W-box DNA chain 2	natural abundance	0.4 ~ 1.0 mM
14	potassium phosphate	natural abundance	20 mM
15	potassium chloride	natural abundance	200 mM
16	zinc chloride	natural abundance	20 uM
17	DTT	[U-2H]	1 mM
18	DSS	natural abundance	50 uM
19	H2O	natural abundance	95 %
20	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
21	WRKY domain	[U-13C; U-15N]	0.4 ~ 1.0 mM
22	W-box DNA chain 1	natural abundance	0.4 ~ 1.0 mM
23	W-box DNA chain 2	natural abundance	0.4 ~ 1.0 mM
24	potassium phosphate	natural abundance	20 mM
25	potassium chloride	natural abundance	200 mM
26	zinc chloride	natural abundance	20 uM
27	DTT	[U-2H]	1 mM
28	DSS	natural abundance	50 uM
29	D2O	natural abundance	100 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
30	WRKY domain	[U-15N]	0.4 ~ 1.0 mM
31	W-box DNA chain 1	[U-15N]-T	0.4 ~ 1.0 mM
32	W-box DNA chain 2	[U-15N]-T	0.4 ~ 1.0 mM
33	potassium phosphate	natural abundance	20 mM
34	potassium chloride	natural abundance	200 mM
35	zinc chloride	natural abundance	20 uM
36	DTT	[U-2H]	1 mM
37	DSS	natural abundance	50 uM
38	D2O	natural abundance	5 %
39	H2O	natural abundance	95 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
40	WRKY domain	[U-15N]	0.4 ~ 1.0 mM
41	W-box DNA chain 1	[U-15N]-T	0.4 ~ 1.0 mM
42	W-box DNA chain 2	[U-15N]-T	0.4 ~ 1.0 mM
43	potassium phosphate	natural abundance	20 mM
44	potassium chloride	natural abundance	200 mM
45	zinc chloride	natural abundance	20 uM
46	DTT	[U-2H]	1 mM
47	DSS	natural abundance	50 uM
48	Pf1 phage	natural abundance	12 mg/mL
49	D2O	natural abundance	5 %
50	H2O	natural abundance	95 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEX, Strand ID: A Detail

Release date

2012-01-17

Citation

Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor
Yamasaki, K., Kigawa, T., Watanabe, S., Inoue, M., Yamasaki, T., Seki, M., Shinozaki, K., Yokoyama, S.
J. Biol. Chem. (2012), 287, 7683-7691, PubMed 22219184 , DOI 10.1074/jbc.M111.279844 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9XI90 released on 2002-05-15
Title WRKY4_ARATH Entity Probable WRKY transcription factor 4

Related entities 1. WRKY domain, : 1 : 1 : 2 : 169 entities Detail

Related entities 3. W-box DNA chain 1, : 2 entities Detail

Related entities 4. W-box DNA chain 2, : 2 entities Detail

Interaction partners 1. WRKY domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-1H NOESY