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Found 1 Document (0.899 seconds)

BMRB: 17735

2LF0

Solution structure of sf3636, a two-domain unknown function protein from Shigella flexneri 2a, determined by joint refinement of NMR, residual dipolar couplings and small-angle X-ray scatting, NESG target SfR339/OCSP target sf3636

Authors

Wu, B., Lemak, A., Yee, A., Lee, H., Gutmanas, A., Semesi, A., Garcia, M., Fang, X., Wang, Y., Prestegard, J.H., Arrowsmith, C.H.

Assembly

sf3636

Entity

1. sf3636 (polymer, Thiol state: not present), 120 monomers, 13726.86 Da Detail

MKEVEKNEIK RLSDRLDAIR HQQADLSLVE AADKYAELEK EKATLEAEIA RLREVHSQKL SKEAQKLMKM PFQRAITKKE QADMGKLKKS VRGLVVVHPM TALGREMGLE EMTGFSKTTF

Formula weight

13726.86 Da

Source organism

Shigella flexneri 2a

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 96.2 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.2 % (1389 of 1444)	96.7 % (740 of 765)	95.5 % (529 of 554)	96.0 % (120 of 125)
Backbone	96.8 % (693 of 716)	97.1 % (236 of 243)	96.9 % (344 of 355)	95.8 % (113 of 118)
Sidechain	96.0 % (809 of 843)	96.6 % (504 of 522)	94.9 % (298 of 314)	100.0 % (7 of 7)
Aromatic	76.0 % (38 of 50)	92.0 % (23 of 25)	60.0 % (15 of 25)
Methyl	97.0 % (128 of 132)	97.0 % (64 of 66)	97.0 % (64 of 66)

1. sf3636

MKEVEKNEIK RLSDRLDAIR HQQADLSLVE AADKYAELEK EKATLEAEIA RLREVHSQKL SKEAQKLMKM PFQRAITKKE QADMGKLKKS VRGLVVVHPM TALGREMGLE EMTGFSKTTF

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	sf3636	[U-100% 13C; U-100% 15N]	0.5 mM
12	TRIS	[U-100% 2H]	10 mM
13	sodium chloride	natural abundance	300 mM
14	zinc sulphate	natural abundance	10 uM
15	DTT	[U-100% 2H]	10 mM
16	NaN3	natural abundance	0.01 %
17	benzamidine	natural abundance	10 mM
18	inhibitor cocktail	natural abundance	1 na
19	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
20	sf3636	[U-7% 13C; U-100% 15N]	0.5 mM
21	TRIS	[U-100% 2H]	10 mM
22	sodium chloride	natural abundance	300 mM
23	zinc sulphate	natural abundance	10 uM
24	DTT	[U-100% 2H]	10 mM
25	NaN3	natural abundance	0.01 %
26	benzamidine	natural abundance	10 mM
27	inhibitor cocktail	natural abundance	1 na
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LF0, Strand ID: A Detail

Release date

2011-07-17

Citation

Solution structure of sf3636, a two-domain unknown function protein from Shigella flexneri 2a, determined by joint refinement of NMR, residual dipolar couplings and small-angle X-ray scatting
Wu, B., Lemak, A., Yee, A., Lee, H., Gutmanas, A., Semesi, A., Garcia, M., Fang, X., Wang, Y., Prestegard, J.H., Arrowsmith, C.H.
Not known

Related entities 1. sf3636, : 1 : 3 entities Detail

Experiments performed 12 experiments Detail

1 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

3 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

4 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

5 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

6 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	sf3636	[U-100% 13C; U-100% 15N]	0.5 mM
12	TRIS	[U-100% 2H]	10 mM
13	sodium chloride	natural abundance	300 mM
14	zinc sulphate	natural abundance	10 uM
15	DTT	[U-100% 2H]	10 mM
16	NaN3	natural abundance	0.01 %
17	benzamidine	natural abundance	10 mM
18	inhibitor cocktail	natural abundance	1 na
19	D2O	natural abundance	100 %

10 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

11 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
20	sf3636	[U-7% 13C; U-100% 15N]	0.5 mM
21	TRIS	[U-100% 2H]	10 mM
22	sodium chloride	natural abundance	300 mM
23	zinc sulphate	natural abundance	10 uM
24	DTT	[U-100% 2H]	10 mM
25	NaN3	natural abundance	0.01 %
26	benzamidine	natural abundance	10 mM
27	inhibitor cocktail	natural abundance	1 na
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

12 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	sf3636	[U-100% 13C; U-100% 15N]	0.7 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

NMR combined restraints 8 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_17735_2lf0.nef
Input source #2: Coordindates	2lf0.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------
QGHMKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVV
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
QGHMKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVV
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

100-------110-------120
HPMTALGREMGLEEMTGFSKTTF
|||||||||||||||||||||||
HPMTALGREMGLEEMTGFSKTTF
-------110-------120---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	123	0	0	100.0

Content subtype: combined_17735_2lf0.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1387		95.9 (chain: A, length: 123)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	3962 (1)	noe	95.9 (chain: A, length: 123)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	112 (1)	hbond	61.0 (chain: A, length: 123)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	200 (200)	.	91.9 (chain: A, length: 123)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	147 (147)	.	82.1 (chain: A, length: 123)
2	RDC restraints	CNS/XPLOR_dipolar_coupling_6	OK	138 (138)	.	77.2 (chain: A, length: 123)
3	RDC restraints	CNS/XPLOR_dipolar_coupling_7	OK	70 (70)	.	56.9 (chain: A, length: 123)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 95.9%
- Total number of assignments
  - ¹H chemical shifts: 739
  - ¹³C chemical shifts: 528
  - ¹⁵N chemical shifts: 120
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	782	739	94.5
¹³C chemical shifts	565	528	93.5
¹⁵N chemical shifts	137	120	87.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	250	235	94.0
¹³C chemical shifts	246	230	93.5
¹⁵N chemical shifts	121	112	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	532	504	94.7
¹³C chemical shifts	319	298	93.4
¹⁵N chemical shifts	16	8	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	73	70	95.9
¹³C chemical shifts	73	70	95.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	23	85.2
¹³C chemical shifts	27	15	55.6

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 95.9%
- Total number of restraints: 3961
- Weight of restraints: 1.0 (3961)
- Potential type of restraints: square-well-parabolic (3961)
- Range of distance target values: 2.25, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 61.0%
  - Total number of restraints: 112
  - Weight of restraints: 1.0 (112)
  - Potential type of restraints: square-well-parabolic (112)
  - Range of distance target values: 1.95, 2.9 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 91.9%
- Total number of restraints: 200
- Total number of restraints per polymer type: 200
- Weight of restraints: 1.0 (200)
- Potential type of restraints: square-well-parabolic (200)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 82.1%

-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------
QGHMKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVV
       |||||  |||||||||||||||| |||||||||| ||||||||||||||||||    |||||||| ||||||||| |||||||||||| ||||
.......EKNEI..LSDRLDAIRHQQADLS.VEAADKYAEL.KEKATLEAEIARLREVHS....KEAQKLMK.PFQRAITKK.QADMGKLKKSVR.LVVV
-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------

100-------110-------120
HPMTALGREMGLEEMTGFSKTTF
| ||||||||||||||||||   
H.MTALGREMGLEEMTGFSK   
100-------110-------

Total number of restraints: 147
Potential type of restraints: undefined (147)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Saveframe: CNS/XPLOR_dipolar_coupling_6

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 77.2%

-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------
QGHMKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVV
       ||||||||||||||||||||||     |||||||||||||||||||||||||     |||||||| ||||||||||||||||||||   ||||
.......EKNEIKRLSDRLDAIRHQQADL.....ADKYAELEKEKATLEAEIARLREVH.....KEAQKLMK.PFQRAITKKEQADMGKLKKS...LVVV
-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------

100-------110-------120
HPMTALGREMGLEEMTGFSKTTF
||| |||||||   ||||||   
HPM.ALGREMG...MTGFSK   
100-------110-------

Total number of restraints: 138
Potential type of restraints: undefined (138)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Saveframe: CNS/XPLOR_dipolar_coupling_7

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 56.9%

-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------
QGHMKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVV
       |  |  | ||||||||| |||      |||| ||||||||||||||||| ||      |  ||||  |||||  | ||  |||||    ||||
.......E..E..R.SDRLDAIRH.QAD......ADKY.ELEKEKATLEAEIARLR.VH......E..KLMK..FQRAI..K.QA..GKLKK....LVVV
-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------

100-------110-------120
HPMTALGREMGLEEMTGFSKTTF
|    |||||     ||||    
H....LGREM.....TGFS    
100-------110------

Total number of restraints: 70
Potential type of restraints: undefined (70)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords two-domain protein

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Found 1 Document (0.899 seconds)

BMRB: 17735

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. sf3636, : 1 : 3 entities Detail

Experiments performed 12 experiments Detail

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NMR combined restraints 8 contents Detail