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BMRB: 17747

2LFC

Solution NMR Structure of Fumarate reductase flavoprotein subunit from Lactobacillus plantarum, Northeast Structural Genomics Consortium Target LpR145J

Authors

Liu, G., Tang, Y., Xiao, R., Janjua, H., Ciccosanti, C., Wang, H., Acton, T., Everett, J.K., Montelione, G.T.

Assembly

LpR145J

Entity

1. LpR145J (polymer, Thiol state: not present), 160 monomers, 18498.54 Da Detail

MVEPGVAKLT TYASKQATDM GAIYVNSKGD RIVNESNVYT TFRNAILKQA DKVAYLVMDE RTWKKVYDLL ILHDFTPEEI KSFFENKGKR PVFVKGSLES AAEQAGIVVD ELVQTVKNYQ GYVQDGHDHD FGRDPKYLHQ FEGETFYIIE QRLEHHHHHH

Formula weight

18498.54 Da

Source organism

Lactiplantibacillus plantarum

Exptl. method

solution NMR

Refine. method

distance geometry, molecular dynamics, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 90.4 %, Completeness (bb): 88.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.4 % (1727 of 1911)	91.2 % (903 of 990)	90.4 % (678 of 750)	85.4 % (146 of 171)
Backbone	88.7 % (844 of 952)	89.0 % (290 of 326)	89.6 % (421 of 470)	85.3 % (133 of 156)
Sidechain	92.3 % (1024 of 1109)	92.3 % (613 of 664)	92.6 % (398 of 430)	86.7 % (13 of 15)
Aromatic	85.3 % (174 of 204)	86.3 % (88 of 102)	84.2 % (85 of 101)	100.0 % (1 of 1)
Methyl	95.9 % (163 of 170)	95.3 % (81 of 85)	96.5 % (82 of 85)

1. LpR145J

MVEPGVAKLT TYASKQATDM GAIYVNSKGD RIVNESNVYT TFRNAILKQA DKVAYLVMDE RTWKKVYDLL ILHDFTPEEI KSFFENKGKR PVFVKGSLES AAEQAGIVVD ELVQTVKNYQ GYVQDGHDHD FGRDPKYLHQ FEGETFYIIE QRLEHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 1.41 mM [U-100% 13C; U-100% 15N]

#	Name	Isotope labeling	Concentration
1	LpR145J	[U-100% 13C; U-100% 15N]	1.41 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 0.9 mM [U-5% 13C; U-100% 15N]

#	Name	Isotope labeling	Concentration
4	LpR145J	[U-100% 13C; U-100% 15N]	0.90 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LFC, Strand ID: A Detail

Release date

2011-08-02

Related entities 1. LpR145J, : 1 : 29 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC