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BMRB: 17750

2LFE

Solution NMR structure of N-terminal domain of human E3 ubiquitin-protein ligase HECW2. Northeast structural genomics consortium (NESG) target ht6306a

Authors

Lemak, A., Yee, A., Houliston, S., Garcia, M., Chitayat, S., Dhe-Paganon, S., Arrowsmith, C.

Assembly

N-terminal domain of E3 ligase HECW2

Entity

1. N-terminal domain of E3 ligase HECW2 (polymer, Thiol state: all free), 138 monomers, 15927.58 Da Detail

MHHHHHHSSQ RENLYFQGLQ RANSDTDLVT SESRSSLTAS MYEYTLGQAQ NLIIFWDIKE EVDPSDWIGL YHIDENSPAN FWDSKNRGVT GTQKGQIVWR IEPGPYFMEP EIKICFKYYH GISGALRATT PCITVKNP

Formula weight

15927.58 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 81.0 %, Completeness (bb): 84.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.0 % (1323 of 1633)	80.7 % (684 of 848)	81.1 % (516 of 636)	82.6 % (123 of 149)
Backbone	84.2 % (685 of 814)	84.5 % (235 of 278)	84.0 % (340 of 405)	84.0 % (110 of 131)
Sidechain	79.0 % (749 of 948)	78.8 % (449 of 570)	79.7 % (287 of 360)	72.2 % (13 of 18)
Aromatic	59.1 % (110 of 186)	60.2 % (56 of 93)	57.3 % (51 of 89)	75.0 % (3 of 4)
Methyl	96.8 % (122 of 126)	96.8 % (61 of 63)	96.8 % (61 of 63)

1. entity

MHHHHHHSSQ RENLYFQGLQ RANSDTDLVT SESRSSLTAS MYEYTLGQAQ NLIIFWDIKE EVDPSDWIGL YHIDENSPAN FWDSKNRGVT GTQKGQIVWR IEPGPYFMEP EIKICFKYYH GISGALRATT PCITVKNP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	N-terminal domain of E3 ligase HECW2	[U-13C; U-15N]	0.5 mM
2	MOPS	natural abundance	10 mM
3	sodium chloride	natural abundance	450 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2)	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.67 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 2LFE, Strand ID: A Detail

Release date

2011-08-02

Citation

NMR solution structure of N-terminal domain of E3 ligase HECW2
Lemak, A., Yee, A., Houliston, S., Garcia, M., Arrowsmith, C., Dhe-Paganon, S.
Not known

Related entities 1. N-terminal domain of E3 ligase HECW2, : 1 : 3 : 13 entities Detail

Interaction partners 1. N-terminal domain of E3 ligase HECW2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 3D HNCO