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BMRB: 17753

2LFH

Solution NMR Structure of the Helix-loop-Helix Domain of Human ID3 Protein, Northeast Structural Genomics Consortium Target HR3111A

Authors

Eletsky, A., Wang, D., Kohan, E., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

HR3111A dimer

Entity

1. HR3111A dimer (polymer, Thiol state: all free), 68 monomers, 7626.609 × 2 Da Detail

MGHHHHHHSH MGGGKGPAAE EPLSLLDDMN HCYSRLRELV PGVPRGTQLS QVEILQRVID YILDLQVV

Total weight

15253.218 Da

Max. entity weight

7626.609 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.5 %, Completeness: 76.7 %, Completeness (bb): 73.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.7 % (594 of 774)	76.9 % (309 of 402)	77.9 % (236 of 303)	71.0 % (49 of 69)
Backbone	73.3 % (293 of 400)	70.5 % (98 of 139)	76.6 % (151 of 197)	68.8 % (44 of 64)
Sidechain	80.7 % (351 of 435)	80.2 % (211 of 263)	80.8 % (135 of 167)	100.0 % (5 of 5)
Aromatic	41.7 % (20 of 48)	41.7 % (10 of 24)	41.7 % (10 of 24)
Methyl	94.9 % (74 of 78)	94.9 % (37 of 39)	94.9 % (37 of 39)

1. HR3111A

MGHHHHHHSH MGGGKGPAAE EPLSLLDDMN HCYSRLRELV PGVPRGTQLS QVEILQRVID YILDLQVV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.9 mM [U-100% 13C; U-100% 15N] HR311A, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR3111A	[U-100% 13C; U-100% 15N]	0.9 mM
2	sodium chloride	natural abundance	100 mM
3	DTT	natural abundance	10 mM
4	TRIS	natural abundance	10 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.5 mM [5% 13C; U-100% 15N] HR311A, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
8	HR3111A	[5% 13C; U-100% 15N]	0.5 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	10 mM
11	TRIS	natural abundance	10 mM
12	sodium azide	natural abundance	0.02 %
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LFH, Strand ID: A, B Detail

Release date

2011-08-02

Related entities 1. HR3111A dimer, : 1 : 4 : 28 entities Detail

Interaction partners 1. HR3111A dimer, : 20 interactors Detail

More details for 20 interactors identified by 7 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC