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BMRB: 17768

2LFP

Structure of bacteriophage SPP1 gp17 protein

Authors

Chagot, B., Auzat, I., Gallopin, M., Petitpas, I., Gilquin, B., Tavares, P., Zinn-Justin, S.

Assembly

gp17

Entity

1. gp17 (polymer, Thiol state: all free), 139 monomers, 15463.31 Da Detail

QGLQTWKLAS RALQKATVEN LESYQPLMEM VNQVTESPGK DDPYPYVVIG DQSSTPFETK SSFGENITMD FHVWGGTTRA EAQDISSRVL EALTYKPLMF EGFTFVAKKL VLAQVITDTD GVTKHGIIKV RFTINNNTG

Formula weight

15463.31 Da

Source organism

Bacillus phage SPP1

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.5 %, Completeness: 64.4 %, Completeness (bb): 69.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.4 % (1041 of 1617)	72.8 % (607 of 834)	50.3 % (319 of 634)	77.2 % (115 of 149)
Backbone	69.7 % (573 of 822)	81.2 % (229 of 282)	57.5 % (234 of 407)	82.7 % (110 of 133)
Sidechain	60.1 % (555 of 924)	66.8 % (369 of 552)	50.8 % (181 of 356)	31.3 % (5 of 16)
Aromatic	70.9 % (95 of 134)	86.6 % (58 of 67)	53.8 % (35 of 65)	100.0 % (2 of 2)
Methyl	58.0 % (94 of 162)	71.6 % (58 of 81)	44.4 % (36 of 81)

1. gp17

QGLQTWKLAS RALQKATVEN LESYQPLMEM VNQVTESPGK DDPYPYVVIG DQSSTPFETK SSFGENITMD FHVWGGTTRA EAQDISSRVL EALTYKPLMF EGFTFVAKKL VLAQVITDTD GVTKHGIIKV RFTINNNTG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	gp17	[U-100% 13C; U-100% 15N]	0.3 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	TRIS	natural abundance	50 mM
5	sodium chloride	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
6	gp17	natural abundance	0.3 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	MES	natural abundance	50 mM
10	sodium chloride	natural abundance	500 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
11	gp17	[U-100% 13C; U-100% 15N]	0.6 mM
12	H2O	natural abundance	90 %
13	D2O	natural abundance	10 %
14	MES	natural abundance	50 mM
15	sodium chloride	natural abundance	500 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LFP, Strand ID: A Detail

Release date

2012-05-08

Citation

Solution structure of gp17 from the Siphoviridae bacteriophage SPP1: insights into its role in virion assembly
Chagot, B., Auzat, I., Gallopin, M., Petitpas, I., Gilquin, B., Tavares, P., Zinn-Justin, S.
Proteins (2012), 80, 319-326, PubMed 22072538 , DOI 10.1002/prot.23191 , Abstract

Related entities 1. gp17, : 1 : 4 : 1 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC