BMRB: 17771

Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of Olfactory Nucleotide Gated Ion Channel

Authors

Deli, I., Chyan, C.

Assembly

CaM-OLFp complex

Entity

1. Calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. OLFp (polymer, Thiol state: not present), 28 monomers, 3325.835 Da Detail

TPRRGRGGFQ RIVRLVGVIR DWANKNFR

3. CA (non-polymer), 40.078 Da

Total weight

20072.125 Da

Max. entity weight

16706.21 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.9 %, Completeness: 91.0 %, Completeness (bb): 97.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.0 % (1838 of 2019)	92.7 % (977 of 1054)	88.5 % (687 of 776)	92.1 % (174 of 189)
Backbone	97.6 % (1025 of 1050)	97.3 % (354 of 364)	97.9 % (502 of 513)	97.7 % (169 of 173)
Sidechain	85.9 % (971 of 1130)	90.3 % (623 of 690)	80.9 % (343 of 424)	31.3 % (5 of 16)
Aromatic	43.2 % (57 of 132)	80.3 % (53 of 66)	4.6 % (3 of 65)	100.0 % (1 of 1)
Methyl	94.7 % (161 of 170)	95.3 % (81 of 85)	94.1 % (80 of 85)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. OLFp

TPRRGRGGFQ RIVRLVGVIR DWANKNFR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 310 (±0.1) K, pH 6.7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]		1 (±0.05) mM
2	OLFp	[U-99% 13C; U-99% 15N]		1 (±0.05) mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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Chem. Shift Complete2

Sequence coverage: 99.4 %, Completeness: 91.0 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.0 % (3676 of 4038)	92.2 % (1943 of 2108)	89.2 % (1385 of 1552)	92.1 % (348 of 378)
Backbone	97.8 % (2054 of 2100)	97.3 % (708 of 728)	98.2 % (1008 of 1026)	97.7 % (338 of 346)
Sidechain	85.8 % (1940 of 2260)	89.5 % (1235 of 1380)	82.0 % (695 of 848)	31.3 % (10 of 32)
Aromatic	42.8 % (113 of 264)	75.0 % (99 of 132)	9.2 % (12 of 130)	100.0 % (2 of 2)
Methyl	96.5 % (328 of 340)	96.5 % (164 of 170)	96.5 % (164 of 170)

1. Calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. OLFp

TPRRGRGGFQ RIVRLVGVIR DWANKNFR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 310 (±0.1) K, pH 6.7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]		1 (±0.05) mM
2	OLFp	[U-99% 13C; U-99% 15N]		1 (±0.05) mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2M0J, Strand ID: A, B Detail

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Release date

2012-09-19

Citation 1

Binding orientation and specificity of calmodulin to rat olfactory cyclic nucleotide-gated ion channel
Irene, D., Huang, J., Chung, T., Li, F., Tzen, J., Lin, T., Chyan, C., Irene, D., Sung, F., Huang, J., Lin, T., Chen, Y., Chyan, C.
J. Biomol. Struct. Dyn. (2013), 31, 414-425, PubMed 22877078 , DOI 10.1080/07391102.2012.703069 , Abstract

Show more 1 citaion

Citation 2

Resonance assignments and secondary structure of calmodulin in complex with its target sequence in rat olfactory cyclic nucleotide-gated ion channel
Biomol. NMR Assign. (2014), 8, 97-102, PubMed 23315338 , DOI 10.1007/s12104-013-9461-y , Abstract

Hide non-primary 1 citaion

Related entities 1. Calmodulin, : 11 : 88 entities Detail

Related entities 2. OLFp, : 1 : 4 : 2 : 7 entities Detail

Interaction partners 2. OLFp, : 1 interactors Detail

Experiments performed 17 experiments Detail