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BMRB: 17780

2LFT

Human prion protein with E219K protective polymorphism

Authors

Biljan, I., Ilc, G., Giancin, G., Zhukov, I., Plavec, J., Legname, G.

Assembly

Human prion protein with E219K protective polymorphism

Entity

1. Human prion protein with E219K protective polymorphism (polymer, Thiol state: all disulfide bound), 142 monomers, 16146.87 Da Detail

GQGGGTHSQW NKPSKPKTNM KHMAGAAAAG AVVGGLGGYM LGSAMSRPII HFGSDYEDRY YRENMHRYPN QVYYRPMDEY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCITQYK RESQAYYQRG SS

Formula weight

16146.87 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS90:SG	1:CYS125:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 88.5 %, Completeness (bb): 82.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.5 % (1445 of 1632)	95.1 % (811 of 853)	78.0 % (486 of 623)	94.9 % (148 of 156)
Backbone	82.9 % (698 of 842)	95.6 % (280 of 293)	69.7 % (287 of 412)	95.6 % (131 of 137)
Sidechain	95.0 % (872 of 918)	94.8 % (531 of 560)	95.6 % (324 of 339)	89.5 % (17 of 19)
Aromatic	89.0 % (137 of 154)	89.6 % (69 of 77)	88.2 % (67 of 76)	100.0 % (1 of 1)
Methyl	98.0 % (100 of 102)	98.0 % (50 of 51)	98.0 % (50 of 51)

1. Hpp E219K

GQGGGTHSQW NKPSKPKTNM KHMAGAAAAG AVVGGLGGYM LGSAMSRPII HFGSDYEDRY YRENMHRYPN QVYYRPMDEY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCITQYK RESQAYYQRG SS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hpp_E219K	[U-100% 13C; U-100% 15N]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	buffer sodium acetate-d3	natural abundance	20 mM
5	NaCl	natural abundance	0.1 M

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
6	Hpp_E219K	[U-100% 13C; U-100% 15N]	1.5 mM
7	D2O	natural abundance	100 %
8	buffer sodium acetate-d3	natural abundance	20 mM
9	NaCl	natural abundance	0.1 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LFT, Strand ID: A Detail

Release date

2012-06-24

Citation

Structural basis for the protective effect of the human prion protein carrying the dominant-negative E219K polymorphism
Biljan, I., Giachin, G., Ilc, G., Zhukov, I., Plavec, J., Legname, G.
Biochem. J. (2012), 446, 243-251, PubMed 22676969 , DOI 10.1042/BJ20111940 , Abstract

Related entities 1. Human prion protein with E219K protective polymorphism, : 1 : 18 : 87 entities Detail

Interaction partners 1. Human prion protein with E219K protective polymorphism, : 73 interactors Detail

More details for 73 interactors identified by 15 citations