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BMRB: 17784

2LFW

NMR structure of the PhyRSL-NepR complex from Sphingomonas sp. Fr1

Authors

Vorholt, J.A., Campagne, S., Damberger, F.F., Allain, F.H-T.

Assembly

PhyRSL-NepR complex

Entity

1. PhyR sigma like domain (polymer, Thiol state: not present), 157 monomers, 17314.20 Da Detail

MSLGQQLAPH LPFLRRYGRA LTGSQNQGDK YVRATLEAIV AAPDQFPRDV DPRLGLYRMF QGIWASANAD GEAQTSQSDA EGTEAVARAR LARMTPLSRQ ALLLTAMEGF SPEDAAYLIE VDTSEVETLV TEALAEIEKQ TRALELVPRG SHHHHHH

2. NepR anti sigma factor (polymer, Thiol state: not present), 62 monomers, 6910.943 Da Detail

MLDLPGNKDK KASSKKSPAK VQSKDRDMGA ALRSAYQKTI EEQVPDEMLD LLNKLALELV PR

Total weight

24225.143 Da

Max. entity weight

17314.2 Da

Source organism

Sphingomonas sp. Fr1

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 89.2 %, Completeness (bb): 93.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (2223 of 2491)	89.5 % (1164 of 1300)	87.2 % (842 of 966)	96.4 % (217 of 225)
Backbone	93.5 % (1206 of 1290)	94.5 % (414 of 438)	91.9 % (593 of 645)	96.1 % (199 of 207)
Sidechain	86.2 % (1214 of 1408)	87.0 % (750 of 862)	84.5 % (446 of 528)	100.0 % (18 of 18)
Aromatic	60.8 % (73 of 120)	76.7 % (46 of 60)	44.1 % (26 of 59)	100.0 % (1 of 1)
Methyl	95.6 % (239 of 250)	96.8 % (121 of 125)	94.4 % (118 of 125)

1. PhyR sigma like domain

MSLGQQLAPH LPFLRRYGRA LTGSQNQGDK YVRATLEAIV AAPDQFPRDV DPRLGLYRMF QGIWASANAD GEAQTSQSDA EGTEAVARAR LARMTPLSRQ ALLLTAMEGF SPEDAAYLIE VDTSEVETLV TEALAEIEKQ TRALELVPRG SHHHHHH

2. NepR anti sigma factor

MLDLPGNKDK KASSKKSPAK VQSKDRDMGA ALRSAYQKTI EEQVPDEMLD LLNKLALELV PR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	PhyR sigma like domain	[U-100% 13C; U-100% 15N]	0.8 (±0.02) mM
2	NepR anti sigma factor	natural abundance	0.8 (±0.02) mM
3	sodium chloride	natural abundance	50 (±0.05) mM
4	sodium phosphate	natural abundance	10 (±0.01) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2LFW, Strand ID: A, B Detail

Release date

2012-04-17

Citation

Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria
Campagne, S., Damberger, F.F., Kaczmarczyk, A., Francez-Charlot, A., Allain, F., Vorholt, J.A.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, E1405-E1414, PubMed 22550171 , DOI 10.1073/pnas.1117003109 , Abstract

Related entities 1. PhyR sigma like domain, : 1 : 12 entities Detail

Related entities 2. NepR anti sigma factor, : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC