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BMRB: 17824

2LGV

Rbx1

Authors

Spratt, D.E., Shaw, G.S.

Assembly

Rbx1

Entity

1. Rbx1 (polymer, Thiol state: all other bound), 100 monomers, 11159.42 Da Detail

GGGGTNSGAG KKRFEVKKSN ASAQSAWDIV VDNCAICRNH IMDLCIECQA NQASATSEEC TVAWGVCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

11355.646 Da

Max. entity weight

11159.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.0 %, Completeness: 72.3 %, Completeness (bb): 76.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.3 % (815 of 1127)	69.1 % (403 of 583)	77.6 % (333 of 429)	68.7 % (79 of 115)
Backbone	76.1 % (455 of 598)	74.4 % (154 of 207)	77.4 % (226 of 292)	75.8 % (75 of 99)
Sidechain	70.0 % (435 of 621)	66.2 % (249 of 376)	79.5 % (182 of 229)	25.0 % (4 of 16)
Aromatic	77.6 % (90 of 116)	77.6 % (45 of 58)	77.8 % (42 of 54)	75.0 % (3 of 4)
Methyl	79.8 % (67 of 84)	78.6 % (33 of 42)	81.0 % (34 of 42)

1. Rbx1

GGGGTNSGAG KKRFEVKKSN ASAQSAWDIV VDNCAICRNH IMDLCIECQA NQASATSEEC TVAWGVCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 15N-sRbx1

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	100 mM
3	DTT	natural abundance	1 mM
4	entity_1	[U-98% 15N]	700 uM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 15N/13C-sRbx1

#	Name	Isotope labeling	Concentration
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	100 mM
9	DTT	natural abundance	1 mM
10	Rbx1	[U-98% 13C; U-98% 15N]	700 uM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 15N/13C-sRbx1

#	Name	Isotope labeling	Concentration
13	sodium phosphate	natural abundance	20 mM
14	sodium chloride	natural abundance	100 mM
15	DTT	natural abundance	1 mM
16	Rbx1	[U-98% 13C; U-98% 15N]	700 uM
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LGV, Strand ID: A Detail

Release date

2012-03-25

Citation

Selective recruitment of an E2~ubiquitin complex by an E3 ubiquitin ligase
Spratt, D.E., Wu, K., Kovacev, J., Pan, Z., Shaw, G.S.
J. Biol. Chem. (2012), 287, 17374-17385, PubMed 22433864 , DOI 10.1074/jbc.M112.353748 , Abstract

Related entities 1. Rbx1, : 1 : 71 : 90 entities Detail

Interaction partners 1. Rbx1, : 76 interactors Detail

More details for 76 interactors identified by 33 citations