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BMRB: 17825

2LGW

Solution Structure of the J Domain of HSJ1a

Authors

Zhou, C., Gao, X., Cao, C., Hu, H.

Assembly

HSJ1a

Entity

1. HSJ1a (polymer, Thiol state: not present), 99 monomers, 11312.34 Da Detail

MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH KREIYDRYGR EPLTGTGTGP SRAEAGSGGP GLEHHHHHH

Formula weight

11312.34 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 73.7 %, Completeness: 52.4 %, Completeness (bb): 59.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.4 % (601 of 1148)	54.9 % (332 of 605)	45.1 % (201 of 446)	70.1 % (68 of 97)
Backbone	59.8 % (348 of 582)	67.5 % (135 of 200)	50.2 % (145 of 289)	73.1 % (68 of 93)
Sidechain	48.7 % (320 of 657)	48.6 % (197 of 405)	49.6 % (123 of 248)	0.0 % (0 of 4)
Aromatic	12.5 % (14 of 112)	25.0 % (14 of 56)	0.0 % (0 of 55)	0.0 % (0 of 1)
Methyl	68.6 % (48 of 70)	68.6 % (24 of 35)	68.6 % (24 of 35)

1. entity

MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH KREIYDRYGR EPLTGTGTGP SRAEAGSGGP GLEHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	HSJ1a	[U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	HSJ1a	[U-100% 13C; U-100% 15N]	1 mM
7	potassium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2LGW, Strand ID: A Detail

Release date

2012-01-08

Citation

The C-terminal helices of heat shock protein 70 are essential for J-domain binding and ATPase activation
Gao, X., Zhou, C., Zhou, Z., Wu, M., Cao, C., Hu, H.
J. Biol. Chem. (2012), 287, 6044-6052, PubMed 22219199 , DOI 10.1074/jbc.M111.294728 , Abstract

Related entities 1. HSJ1a, : 1 : 2 : 37 entities Detail

Interaction partners 1. HSJ1a, : 15 interactors Detail

More details for 15 interactors identified by 10 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC