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BMRB: 17827

2LGX

1H, 13C, and 15N Chemical Shift Assignments for Kindle-2 N-terminus

Authors

Perera, H.DHANUJA., Ma, Y., Yang, J., Hirbawi, J., Plow, E.F., Qin, J.

Assembly

k2-n

Entity

1. k2-n (polymer, Thiol state: all free), 112 monomers, 12967.83 Da Detail

GAMPDEFMAL DGIRMPDGCY ADGTWELSVH VTDVNRDVTL RVTGEVHIGG VMLKLVEKLD VKKDWSDHAL WWEKKRTWLL KTHWTLDKYG IQADAKLQFT PQHKLLRLQL PN

Formula weight

12967.83 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.4 %, Completeness: 72.6 %, Completeness (bb): 85.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.6 % (981 of 1351)	68.0 % (476 of 700)	75.7 % (402 of 531)	85.8 % (103 of 120)
Backbone	85.2 % (566 of 664)	83.8 % (191 of 228)	85.4 % (280 of 328)	88.0 % (95 of 108)
Sidechain	64.0 % (506 of 791)	60.4 % (285 of 472)	69.4 % (213 of 307)	66.7 % (8 of 12)
Aromatic	16.4 % (21 of 128)	23.4 % (15 of 64)	0.0 % (0 of 58)	100.0 % (6 of 6)
Methyl	89.7 % (122 of 136)	89.7 % (61 of 68)	89.7 % (61 of 68)

1. entity

GAMPDEFMAL DGIRMPDGCY ADGTWELSVH VTDVNRDVTL RVTGEVHIGG VMLKLVEKLD VKKDWSDHAL WWEKKRTWLL KTHWTLDKYG IQADAKLQFT PQHKLLRLQL PN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	k2-n	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	k2-n	natural abundance	1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	sodium azide	natural abundance	0.05 %
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	k2-n	[U-100% 15N]	0.1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LGX, Strand ID: A Detail

Release date

2011-11-30

Citation

Membrane binding of the N-terminal ubiquitin-like domain of kindlin-2 is crucial for its regulation of integrin activation
Perera, H.DHANUJA., Ma, Y., Yang, J., Hirbawi, J., Plow, E.F., Qin, J.
Structure (2011), 19, 1664-1671, PubMed 22078565 , DOI 10.1016/j.str.2011.08.012 , Abstract

Related entities 1. k2-n, : 1 : 32 entities Detail

Interaction partners 1. k2-n, : 14 interactors Detail

More details for 14 interactors identified by 7 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC