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BMRB: 17833

2N4I

Skint1 IgV

Authors

Salim, M., Knowles, T.J., Willcox, C., Mohammed, F., Woodard, M., Overduin, M., Hayday, A.C., Willcox, B.E.

Assembly

Skint1 IgV

Entity

1. Skint1 IgV (polymer, Thiol state: all disulfide bound), 238 monomers, 27090.29 Da Detail

MSSEPFIVNG LEGPVLASLG GNLELSCQLS PPQQAQHMEI RWFRNLYTEP VHLYRDGKDM FGEIISKYVE RTELLKDGIG EGKVTLRIFN VTVDDDGSYH CVFKDGDFYE EHITEVKITM SSEPFIVNGL EGPVLASLGG NLELSCQLSP PQQAQHMEIR WFRNLYTEPV HLYRDGKDMF GEIISKYVER TELLKDGIGE GKVTLRIFNV TVDDDGSYHC VFKDGDFYEE HITEVKIT

Formula weight

27090.29 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS27:SG	1:CYS101:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 57.6 %, Completeness: 46.1 %, Completeness (bb): 54.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.1 % (1289 of 2798)	43.9 % (638 of 1452)	47.7 % (525 of 1100)	51.2 % (126 of 246)
Backbone	54.3 % (765 of 1408)	52.0 % (254 of 488)	55.9 % (387 of 692)	54.4 % (124 of 228)
Sidechain	40.1 % (644 of 1606)	39.8 % (384 of 964)	41.3 % (258 of 624)	11.1 % (2 of 18)
Aromatic	3.1 % (8 of 256)	3.1 % (4 of 128)	3.2 % (4 of 126)	0.0 % (0 of 2)
Methyl	52.0 % (133 of 256)	51.6 % (66 of 128)	52.3 % (67 of 128)

1. Skint1 IgV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Skint1 IgV	[U-100% 13C; U-100% 15N]	1.4 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N4I, Strand ID: A Detail

Release date

2016-02-28

Citation

Characterisation of a putative receptor binding surface on Skint-1, a critical determinant of dendritic epidermal T cell selection
Salim, M., Knowles, T.J., Willcox, C., Mohammed, F., Woodard, M., Overduin, M., Hayday, A.C., Willcox, B.E.
J. Biol. Chem. (2016), PubMed , DOI:

Related entities 1. Skint1 IgV, : 109 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC