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BMRB: 17842

2LHF

Solution structure of outer membrane protein H (OprH) from P. aeruginosa in DHPC micelles.

Authors

Edrington, T.C., Tamm, L.K.

Assembly

OprH

Entity

1. OprH (polymer, Thiol state: all free), 179 monomers, 19529.30 Da Detail

ADNFVGLTWG ETSNNIQKSK SLNRNLNSPN LDKVIDNTGT WGIRAGQQFE QGRYYATYEN ISDTSSGNKL RQQNLLGSYD AFLPIGDNNT KLFGGATLGL VKLEQDGKGF KRDSDVGYAA GLQAGILQEL SKNASIEGGY RYLRTNASTE MTPHGGNKLG SLDLHSSSQF YLGANYKFL

Formula weight

19529.3 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 43.1 %, Completeness (bb): 76.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.1 % (878 of 2037)	21.6 % (229 of 1058)	63.4 % (491 of 774)	77.1 % (158 of 205)
Backbone	76.0 % (812 of 1068)	47.6 % (180 of 378)	92.4 % (475 of 514)	89.2 % (157 of 176)
Sidechain	18.3 % (206 of 1125)	7.2 % (49 of 680)	37.5 % (156 of 416)	3.4 % (1 of 29)
Aromatic	0.0 % (0 of 174)	0.0 % (0 of 87)	0.0 % (0 of 85)	0.0 % (0 of 2)
Methyl	18.0 % (31 of 172)	12.8 % (11 of 86)	23.3 % (20 of 86)

1. entity

ADNFVGLTWG ETSNNIQKSK SLNRNLNSPN LDKVIDNTGT WGIRAGQQFE QGRYYATYEN ISDTSSGNKL RQQNLLGSYD AFLPIGDNNT KLFGGATLGL VKLEQDGKGF KRDSDVGYAA GLQAGILQEL SKNASIEGGY RYLRTNASTE MTPHGGNKLG SLDLHSSSQF YLGANYKFL

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.1, Details 150 mM DHPC

#	Name	Isotope labeling	Concentration
1	OprH	[U-13C; U-15N; U-2H]	1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.05 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LHF, Strand ID: A Detail

Release date

2011-08-24

Citation

Structural basis for the interaction of lipopolysaccharide with outer membrane protein H (OprH) from Pseudomonas aeruginosa
Edrington, T.C., Kintz, E., Goldberg, J.B., Tamm, L.K.
J. Biol. Chem. (2011), 286, 39211-39223, PubMed 21865172 , DOI 10.1074/jbc.M111.280933 , Abstract

Related entities 1. OprH, : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC-TROSY